Existence of Yersinia species in marketable milk and some dairy products | ||
| New Valley Veterinary Journal | ||
| Article 3, Volume 2, Issue 2, June 2022, Pages 17-20 PDF (975.26 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/nvvj.2022.153536.1008 | ||
| Authors | ||
| Maha Hamed* ; Nagah Saad; Wallaa Amin | ||
| Department of Food Hygiene (Milk hygiene), Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt. | ||
| Abstract | ||
| Background and aims: Yersinia species are members of the Enterobacteriaceae. These bacteria have Gram-negative or Gram-variable, rod-shaped cells. Yersinia regards as one of the most important food-borne agents along with ability to grow at room and refrigerator temperatures. There are several intestinal and extraintestinal clinical symptoms caused by these organisms, appearing through mild gastroenteritis, mesenteric lymphadenitis and pseudo appendicitis. Yersiniosis is a zoonotic disease with a wide distribution and a known public health significance caused by Y. enterocolitica . Materials and Methods: One hundred and sixty marketable milk samples and some milk products including kareish cheese, Domiati cheese and small scale ice cream were collected randomly from supermarkets, dairy farms, dairy shops and street vendors, in Assiut city, Egypt. Selective CIN (Cefsulodin-Irgasan-Novobiocin) agar plates were used after enriched by phosphate buffered saline (PBS) supplemented with novobiocin and cefsulodin and incubated at 30 °C for 24 to 48 hours. The suspected colonies were identified by biochemical tests. Results: A total of 33 isolates (20.63%) were identified as Yersinia species, representing Y. enterocolitica (47.5%), Y. pseudotuberculosis (7.5%), Y. pestis (17.5%) and Y. intermedia (10%). | ||
| Keywords | ||
| Yersinia species; marketable milk; cheese; small scale ice cream; selective media (CIN) | ||
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