Histological evaluation of vitamin-D deficiency on the endometrial decidualization in early pregnancy in rat model | ||
| Egyptian Journal of Histology | ||
| Article 6, Volume 41, Issue 3, September 2018, Pages 300-310 PDF (4.13 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejh.2018.3665.1007 | ||
| Author | ||
| Samaa Samir Kamar | ||
| Department of Medical Histology, Faculty of Medicine, Cairo University, Cairo, Egypt. | ||
| Abstract | ||
| ABSTRACT Introduction: Vitamin-D is a pleiotropic secosteroid hormone that exerts classical effect in calcium uptake and bone metabolism. In addition, it has non-classical effects on variety of organs. Endometrial decidualization, placentation, and the development of the embryo itself have all pivotal roles for normal pregnancy outcomes after embryo implantation. Early maternal decidua and fetal trophoblast cells illustrate expression of vitamin-D receptor and 1α-hydroxylase enzyme that catalyzes vitamin-D into the active metabolite, calcitriol. Aim: To record the histological effect of vitamin-D deficiency on endometrial decidualization during early pregnancy on day 8 post-coitum. Materials and Methods: Thirty female albino rats were divided into 2 groups: control group (GpI) and vitamin-D deficient group (GpII). After establishment of pregnancy, the animals were sacrificed on the 8th gestational day. The bicornuate pregnant uteri were processed for histological analysis using H&E and PAS stains in addition to immunohistochemical staining for osteopontin (OSP), as a marker for decidualization, and for CD34 to assess the decidual angiogenesis. Morphometric and statistical studies were performed. Results: Substantial decrease in the number of implantation sites in vitamin-D deficient rats was noted by gross examination. Histological examination revealed significant decrease in endometrial thickness at the antimesometrial region with remarkably impaired stromal cells differentiation. PAS staining illustrated fewer PAS positively stained uterine natural killer cells compared to control. Weak cytoplasmic OSP immunostaining was observed in the luminal as well as glandular epithelium, vascular endothelium and few stromal cells in mesometrial region. CD34 immunostaining demonstrated impaired angiogenesis in the lateral decidua as compared to control. These findings were confirmed by morphometric and statistical analysis. Conclusion: Vitamin-D deficiency impairs the uterine stromal cells differentiation, affects the immunity tolerance and weakens decidual angiogenesis during early pregnancy in rat model. | ||
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