El Sheikh, I., elhawary, S., Tadros, S., Nazif, N., AbdelMohsen, M., Taha, H. (2023). In Vitro Propagation and Evaluation of Valtrate and Valereinic Acid in Valerina Officinalis L.. test, 66(7), 381-391. doi: 10.21608/ejchem.2022.151906.6603
Iman El Seody El Sheikh; seham Salah elhawary; Soad Hanna Tadros; Naglaa Mohamed Nazif; Mona Mohamed AbdelMohsen; Hussein S. Taha. "In Vitro Propagation and Evaluation of Valtrate and Valereinic Acid in Valerina Officinalis L.". test, 66, 7, 2023, 381-391. doi: 10.21608/ejchem.2022.151906.6603
El Sheikh, I., elhawary, S., Tadros, S., Nazif, N., AbdelMohsen, M., Taha, H. (2023). 'In Vitro Propagation and Evaluation of Valtrate and Valereinic Acid in Valerina Officinalis L.', test, 66(7), pp. 381-391. doi: 10.21608/ejchem.2022.151906.6603
El Sheikh, I., elhawary, S., Tadros, S., Nazif, N., AbdelMohsen, M., Taha, H. In Vitro Propagation and Evaluation of Valtrate and Valereinic Acid in Valerina Officinalis L.. test, 2023; 66(7): 381-391. doi: 10.21608/ejchem.2022.151906.6603

In Vitro Propagation and Evaluation of Valtrate and Valereinic Acid in Valerina Officinalis L.

Egyptian Journal of Chemistry
Volume 66, Issue 7, July 2023, Pages 381-391    PDF (803.77 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.151906.6603
Authors
Iman El Seody El Sheikh* 1; seham Salah elhawary2; Soad Hanna Tadros3; Naglaa Mohamed Nazif4; Mona Mohamed AbdelMohsen4; Hussein S. Taha5
1Chemistry of Medicinal Plants Department, Division of Pharmaceutical and Drug Industries, National Research Centre, Buhouth St. (Former El Tahrir St.), 12622, Dokki, Giza, Egypt.
2department of pharmacognosy, faculty of pharmacy, Cairo university
3Pharmacognosy Department, Faculty of Pharmacy. Cairo University
4aChemistry of Medicinal Plants Department, Division of Pharmaceutical and Drug Industries, National Research Centre, Buhouth St. (Former El Tahrir St.), 12622, Dokki, Giza, Egypt.
5Plant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Centre (NRC), Dokki, Cairo, Egypt
Abstract
Valeriana officinalis L is the most cost-effective plant in Europe. Due to its cardiovascular, anxiolytic, sedative, and anticonvulsant properties, the root of Valeriana is extensively employed in herbal therapies. Seeds of Valeriana were germinated in vitro by soaking for 24 hours in 150 ppm Gibberellic acid (GA3) further, cultivated in half MS (Murashige and Skoog salts medium with vitamins)medium. Treatment of MS media with 5 ppm of Benzyl amino purine (BA) and 2,4-Dichlorophenoxy acetic acid (2,4-D) increased fresh and dry weights of calli produced from leaf explants. However, supplemented MS media with 3 ppm BA and 1 ppm 2,4-D enhanced the efficiency rate of regeneration. On the other hand, augmentation of MS-medium with 0.1 ppm Indole-3-butyric acid(IBA), achieved of rooting process, while acclimatization was accomplished by using peat moss:vermiculite (1:1). Valerenic acid and valtrate were analyzed qualitatively and quantitatively by HPLC in mother plant, callus, regenerated shoots, roots, and Dormival medication which used as a control. The findings showed that regenerated shoots had the greatest concentration of valerenic acid across various extracts (3.80±0.82mg/g DW), whereas in vitro-derived roots from leaf explants contained the highest concentration of valtrate (3.82±0.23mg/g DW).
Keywords
Valeriana officinalis L; Callus; Regeneration; Roots; Acclimatization; HPLC; Valerenic acid and valtrate
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