Bone Marrow –Derived Mesenchymal Stem Cells and Their Therapeutic Role in Type 1 Diabetes Mellitus | ||
| Suez Canal Veterinary Medical Journal. SCVMJ | ||
| Articles in Press, Accepted Manuscript, Available Online from 17 October 2022 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/scvmj.2022.160652.1101 | ||
| Authors | ||
| Amira E. Khorshed* 1; Hussein Eidaroos2; Sameh M. Farouk3; Rania A. Galhom4 | ||
| 1Department of Cytology &Histology, Faculty of Vet. Med., Suez Canal University, Ismailia, Egypt | ||
| 2Department of Cytology & Histology, Faculty of Vet. Med., Suez Canal University | ||
| 3Department of Cytology and Histology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt | ||
| 4Department of Human Anatomy and embryology, Faculty of Medicine, Suez Canal University. Tissue culture unite, Center of Excellence in Molecular and Cellular Medicine (CEMCM), Faculty of medicine, Suez Canal University. Department | ||
| Abstract | ||
| Diabetes Mellitus (DM) is the most widespread endocrine disease characterized by hyperglycemia, impaired carbohydrate, lipid, and protein metabolism. Recently, numerous research focused on the potential application of adult mesenchymal stem cells (MSCs) as an alternative therapeutic for diabetes treatment owing to the diverse properties of these cells with hopeful results. Thus, the present study aimed to investigate the potential of type 1 diabetes mellitus (T1DM) recovery through the engraftment of undifferentiated MSCs, as well as the impact on the pancreatic β cells. The experiment was carried out on thirty adult male and female albino rats weighting (180-200 gm each). Twenty-five female rats used in the experiments was divided into 3 groups; control group (5 rats), diabetic group injected intra-peritoneal with 60mg/kg streptozotocin (STZ) (10 rats), and diabetic treated with 3×106 of undifferentiated male bone marrow MSCs) BM-MSCs) I/V single dose (10 rats). BM-MSCs were isolated (from 5 male donor rats), cultured, and characterized by flow cytometry. The pancreatic tissues from all experimental groups were removed and prepared for histological, histochemical, immunohistochemical, molecular as well as morphometric studies. The obtained results were showed that the diabetic untreated animals had vacuolations, reduction in size of pancreatic islets, and congestion in blood vessels. Moreover, positive PCR product of Sry gene, highly significant increase in insulin immunoreactivity, and restoration the normal morphology of pancreatic islets in diabetic group treated with BM-MSCs. | ||
| Keywords | ||
| Blood vessels; Diabetes Mellitus; Mesenchymal stem cells; Pancreas; STZ | ||
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