Extracellular lipase production by local isolate of Penicillium citrinum | ||
| Al-Azhar Journal of Agricultural Research | ||
| Article 8, Volume 47, Issue 1, June 2022, Pages 68-78 PDF (562.88 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ajar.2022.266485 | ||
| Authors | ||
| H. S. El-Shall1; A. A. Shoukry1; M. H. Abd El-Aleim2; H. H. Elsebaay1 | ||
| 1Agriculture Botany Department (Microbiology), Faculty of Agriculture, AL-Azhar University Cairo, Egypt. | ||
| 2Department of Microbiology, Faculty of Agriculture, Cairo University. Giza, Egypt. | ||
| Abstract | ||
| This study investigated the screening, production and optimization of extracellular lipase from a fungus isolated from different samples in Egypt. The isolated fungi were screened on tributyrin agar for exogenous lipolytic activity. A promising lipase producing isolate of SH5Penicillium citrinum was selected and identified by the isolate SH5 of Penicillium citrinum that was identified as the highest lipase-producing strain. Molecular identification was carried out by amplifications of ITS-1, 5.8S and ITS-2 regions. The effects of incubation time, inducers, pH, temperature, carbon and nitrogen sources were varied for optimal lipase production using one factor at a time approach. Maximum lipase production was observed on the third day, pH 7.0 and at 30oC. Olive oil, maltose and ammonium sulphate were the best inducers, carbon and nitrogen sources were respectively for lipase production. | ||
| Keywords | ||
| Lipase; Extracellular enzyme; Optimization; Fermentation; Penicillium citrinum | ||
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