Dorucu, M., Ispir, U., Colak, S., Altinterim, B., Celayir, Y. (2009). The Effect of Black Cumin Seeds, Nigella sativa, on the Immune Response of Rainbow Trout, Oncorhynchus mykiss. test, 2(1), 27-33. doi: 10.21608/maj.2009.2667
M. Dorucu; U. Ispir; S. Colak; B. Altinterim; Y. Celayir. "The Effect of Black Cumin Seeds, Nigella sativa, on the Immune Response of Rainbow Trout, Oncorhynchus mykiss". test, 2, 1, 2009, 27-33. doi: 10.21608/maj.2009.2667
Dorucu, M., Ispir, U., Colak, S., Altinterim, B., Celayir, Y. (2009). 'The Effect of Black Cumin Seeds, Nigella sativa, on the Immune Response of Rainbow Trout, Oncorhynchus mykiss', test, 2(1), pp. 27-33. doi: 10.21608/maj.2009.2667
Dorucu, M., Ispir, U., Colak, S., Altinterim, B., Celayir, Y. The Effect of Black Cumin Seeds, Nigella sativa, on the Immune Response of Rainbow Trout, Oncorhynchus mykiss. test, 2009; 2(1): 27-33. doi: 10.21608/maj.2009.2667

The Effect of Black Cumin Seeds, Nigella sativa, on the Immune Response of Rainbow Trout, Oncorhynchus mykiss

Mediterranean Aquaculture Journal
Article 4, Volume 2, Issue 1, January 2009, Pages 27-33    PDF (375.99 K)
Document Type: Original Article
DOI: 10.21608/maj.2009.2667
Authors
M. Dorucu1; U. Ispir1; S. Colak2; B. Altinterim1; Y. Celayir3
1Firat University, Fisheries Faculty, 23119 Elazığ, TURKIYE
2Istanbul University, Fisheries Faculty Ordu Cad. No: 200 34470 Eminonu, Istanbul, TURKIYE and
3The State Hydraulic Works, IX. Region Directorate, 23700 Keban, Elazig, TURKIYE
Abstract
Using of natural plants as immunostimulant in fish is more useful than antibacterial drugs that cause adversely side effects for fish, environment and consumers. Therefore, we investigated the effect of black cumin seeds, Nigella sativa, as a herbal medicine plant on some immuno-hematological parameters and specific as well as non-specific defence mechanisms of rainbow trout, Oncorhynchus mykiss. Fish were fed with basal diet incorporated with 1, 2.5 and 5% Nigella sativa for 21 days. At the end of experiment, the hematocrit (Hct), leukocyte levels, glass-adherent NBT positive cell activation, serum protein and total immunoglobulin level were determined in fish blood. No significant differences (P >0.05) were found in hematocrit levels of fish fed the diets containing 1 and 2.5% black cumin seeds, but it was significantly (P <0.05) increased in fish receiving diet containing 5% black cumin seeds compared to the control. Glass-adherent NBT positive cell activation and leukocyte level in black cumin supplemented groups showed no significant differences (P >0.05) when compared to the control group. The serum protein and total immunoglobulin levels were significantly (P <0.05) higher than those of the control group. In conclusion, based on the current findings together with the low cost and the immunostimulative effect of black cumin seed, it is recommended to be used in fish feed to minimize the mortalities caused by some pathogens.
Keywords
Black cumin seed; Nigella Sativa; Rainbow trout; Specific immunity; Non-specific immunity
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