Antioxidant and Anticholinesterase Effects of Methanol Extract, and Consecutive Fractions of Peganum harmala L. | ||
| Egyptian Journal of Chemistry | ||
| Article 43, Volume 66, Issue 6, June 2023, Pages 431-440 PDF (975.22 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2022.151986.6663 | ||
| Authors | ||
| Amel Nait Marzoug* 1; Fadila Khaldi1; Adel Ayari1; Lynda Gali2; Abdelhak Gheid3 | ||
| 1Department of Biology, Faculty of Sciences and Nature, University Mohamed Cherif Messaadia. Souk-Ahras, Algeria | ||
| 2Biotechnology Research Center, Constantine, Algeria | ||
| 3Department of Matter Sciences, Faculty of Science and Technology, University Mohamed Cherif Messaadia. Souk-Ahras, Algeria | ||
| Abstract | ||
| Peganum harmala L. is a medicinal plant growing in the region of Sidi Fredj of Souk Ahras city (Northeastern of Algeria), whose native populations commonly used this plant to treat various health conditions and illnesses. Up to now, no previous study on the beneficial effects of P.harmala in the Souk Ahras region has been conducted. Therefore, the present study aimed to investigate the phenolic and flavonoid composition, as well as the antioxidant activities of the methanol extract and its three fractions: chloroform, ethyl acetate and butanol from seeds of P. harmala using eight different methods. The inhibitory effect of the extracts against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) was also determined. The results showed that the ethyl acetate fraction contained the highest amounts of total phenolic and total flavonoid (237.23±1.06 µg GAE/mg and 82.58±0.03 µg QE/mg, respectively). Important antioxidant activity was exerted by the extracts, especially the ethyl acetate fraction, which was the most effective. However, a weak antioxidant activity in Ferrous ions chelation was observed for all extracts with >800 µg/mL. The samples showed also a strong inhibitory effect against AChE and BChE, the chloroform fraction was the most potent with IC50 values of 14.69±0.10 μg/mL against AChE and 7.36±0.77 μg/mL against BChE comparable to that of galantamine. Conclusively, the obtained results proved the beneficial antioxidant and anticholinesterase activities of P. harmala-derived bioactive molecules. | ||
| Keywords | ||
| Peganum harmala L; antioxidant & anticholinesterase effects; phenolic compounds; flavonoids | ||
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