Intestinal Mucositis Induced by 5- Fluorouracil and the Possible Protective Effect of Probiotics Containing Lactobacillus in Adult Male Albino Rats: Histological and Immunohistochemical Study | ||
| Benha Medical Journal | ||
| Article 2, Volume 40, Special issue (Academic), September and October 2023, Pages 17-34 PDF (1.84 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bmfj.2022.153141.1630 | ||
| Authors | ||
| Nehal Fahmy Shaheen* 1; Hend Ragab Mousa2 | ||
| 1Anatomy and Embryology Department ,Benha faculty of medicine, Benha university | ||
| 2Anatomy and Embryology Department ,Benha faculty of mecidine ,Benha university | ||
| Abstract | ||
| Introduction: Intestinal mucositis is the most prevalent side effect of antineoplastic drug 5-flurouoracil (5-FU). Probiotics are live microorganisms that improve intestinal immune regulation. So, probiotics might have a beneficial effect to reduce the chemotherapy-induced enterotoxicity. Aim: This study was designed to assess mucositis induced by 5-FU and the possible protective effect of probiotics Methods: 50 healthy albino adult male rats were divided into five equivalent groups. Control group, Probiotic group was given 100 µL of suspension containing 1x107 ( CFU) of probiotic cocktail daily.5-FU group was received 50 mg/kg B.W. of 5-FU by intraperitoneal (IP) injection once daily for 5 consecutive days. Probiotic plus 5-FU group was given 50 mg/kg B.W. of 5-FU by IP injection for 5 consecutive days with probiotic cocktail once daily for 9 consecutive days. Recovery group was received 50 mg/kg body weight 5-FU by IP injection once daily for 5 consecutive days then left without medication till rats had been sacrificed on 9th day .The intestinal tissues have been processed for a light microscopic study. Results: Rats of 5-FU group showed broadening, fusion and sloughing of villi into lumen, ulcerations, marked depletion of goblet cells, strong iNOS, marked caspase 3 and weak Ki-67 immunoreactions . Rats treated with 5-FU plus probiotic showed preservation of normal intestinal architecture with improvement of histological and immunohistological features of mucositis . Rats of recovery group showed decrease histological and immunohistochemical features of mucositis. Conclusion: oral intake of the probiotic can protect against chemotherapy-causing intestinal mucositis in a rat model. | ||
| Keywords | ||
| 5-flurouoracil; probiotic; mucositis | ||
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