Radiation-induced effects on genes regulating DNA Repair and Cell Cycle in breast cancer patients. | ||
| Zagazig University Medical Journal | ||
| Article 25, Volume 30, Issue 1.4, May and June 2024, Pages 275-285 PDF (738.88 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/zumj.2022.165257.2649 | ||
| Authors | ||
| Sameh Fawzy Nakhla* 1; Rana Khafaga2; Marwa Gaber3; Tarek Aref4; Medhat Anwar5; Shaza Saadi6; Esraa Mohamed7; Esraa Gad7; Amr Hussien8; Abdelsalam Ismail9; Sawsan Moussa10 | ||
| 1Radiation Sciences department, Medical Research Institute (MRI), Alexandria University, Alexandria, Egypt. | ||
| 2Department of Biochemistry, Medical Research Institute, Alexandria University. | ||
| 3Department of Eperimental and Clinical internal Medicine, Medical Research Institute, Alexandria University. | ||
| 4Department of Radiology, Medical Research Institute, Alexandria University | ||
| 5Department of Experimental and Clinical Surgery, Medical Research Institute, Alexandria University | ||
| 6Department of Radiology and Medical Imaging, Faculty of Medical Applied Sciences, Al-Azhar University- Palestine | ||
| 7Department of Radiology, faculty of Applied Medical Science, October 6 University. | ||
| 8Department of Cancer management and research, Medical Research Institute, Alexandria University | ||
| 9Department of clinical oncology, Faculty of Medicine, Alexandria University | ||
| 10Department of Radiation Science, Medical Research Institute, Alexandria University | ||
| Abstract | ||
| Background: Ionizing radiation is a major DNA damaging agent. One of the most common sources of exposure is through medical diagnostics or treatments such as cancer radiotherapy. Aim: This study aims to assess the radiation-induced changes in the expression of DNA repair and cell cycle regulation genes (POLH, PCNA, DDB2, and XPC) in the blood of breast cancer patients and to evaluate their potential as predictive biomarkers for treatment responses. Methods: In this study, the levels of circulating PCNA, POLH, XPC, and DDB2 were evaluated in 51 females: 31 cancer patients and 20 healthy volunteers as a control group. The genes were extracted from whole blood samples and cDNA was synthesized; RT-qPCR was used to assay the expression pattern of these genes. Results: Circulating DDB2 showed a significant difference in the relative expression among the three studied groups. Also, there was a significant difference in the relative expression of circulating DDB2 in the pre-radiotherapy and post-radiotherapy groups relative to the control group. Also, relative to the control group, the circulating levels of the four genes were higher in the pre-radiotherapy group to about double that in the post-radiotherapy group. The four genes combination was capable to discriminate the pre-radiotherapy group from the post-radiotherapy group significantly. Conclusion: The relative expression of these four genes as radiation-responsive genes that are involved in cell cycle regulation and DNA repair could be changed due to radiotherapy. Also, their circulating levels in breast cancer patients could be promising predictive biomarkers for radiotherapy responses. | ||
| Keywords | ||
| Breast Cancer; radiation-responsive genes; radiotherapy | ||
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