Khashaba, R., Abdelsamee, S., Omar, F., El shimi, O. (2022). The Role of Serum microRNA-1165-3p in Allergic Asthma. test, 39(3), 924-932. doi: 10.21608/bmfj.2022.161982.1665
Rana Atef Khashaba; Soher Abdelrahman Abdelsamee; fatma Reda Omar; Ola Samir El shimi. "The Role of Serum microRNA-1165-3p in Allergic Asthma". test, 39, 3, 2022, 924-932. doi: 10.21608/bmfj.2022.161982.1665
Khashaba, R., Abdelsamee, S., Omar, F., El shimi, O. (2022). 'The Role of Serum microRNA-1165-3p in Allergic Asthma', test, 39(3), pp. 924-932. doi: 10.21608/bmfj.2022.161982.1665
Khashaba, R., Abdelsamee, S., Omar, F., El shimi, O. The Role of Serum microRNA-1165-3p in Allergic Asthma. test, 2022; 39(3): 924-932. doi: 10.21608/bmfj.2022.161982.1665

The Role of Serum microRNA-1165-3p in Allergic Asthma

Benha Medical Journal
Article 15, Volume 39, Issue 3, November and December 2022, Pages 924-932    PDF (779.67 K)
Document Type: Original Article
DOI: 10.21608/bmfj.2022.161982.1665
Authors
Rana Atef Khashaba* 1; Soher Abdelrahman Abdelsamee2; fatma Reda Omar3; Ola Samir El shimi4
1الشیخ زاید مدینة الخمایل المرحلة الأولى أمام هایبر وان بوابة 2 عمارة 120 شقة 1
2Clinical pathology department, faculty of medicine, benha university, Egypt
3M.B.B.Ch, faculty of medicine, Tanta university, Egypt
4clinical & chemical pathology department,faculty of medicine, Benha university, Egypt.
Abstract
Background: Asthma is a complex, multifactorial, immune-mediated condition with a number of molecular immunopathological mechanisms underlying airway inflammation, hyperreactivity, and bronchial remodeling. MicroRNAs are important regulators in the pathogenesis and progression of chronic respiratory diseases, including bronchial asthma. Objective: The aim of the study is to investigate the level of expression of serum miR-1165-3p in allergic asthma and its correlation with serum IgE levels. Subjects and Methods: A case-control study was conducted on 30 allergic asthma cases and 20 healthy subjects as a control group. The level of expression miR-1165-3p was evaluated by qRT-PCR using the comparative threshold cycle (Ct) method. Serum total IgE levels were measured using enzyme-linked immunosorbent assay (ELISA). Results: miR-1165-3p and serum total IgE level were significantly upregulated and elevated, respectively, in asthmatic patients in comparison with control group. The area under the ROC curve of miR-1165-3p showed 0.988 (95% CI 0.967 to 1, P value < 0.001). Asthmatic patients showed a significant positive correlation between the expression level of miR-1165-3p and IgE levels (r =0.687, P
Keywords
Asthma; miRNA; miR-1165-3p; biomarker
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