Mahmood, D., Osman, Y., Nidhamaddin, S., Ahmadissa, S., Rahim, A., Salih, M., Hamasoor, T. (2022). Identification of Candida Species Isolated from Oral Candidiasis in Cancer Patients using PCR-RFLP in Sulaimani, Iraq. test, 14(2), 115-126. doi: 10.21608/eajbse.2022.269215
Diman M. Mahmood; Yad N. Osman; Sara J. Nidhamaddin; Solhan M. Ahmadissa; Alaa A. Rahim; Mariwan A. Salih; Taib A. Hamasoor. "Identification of Candida Species Isolated from Oral Candidiasis in Cancer Patients using PCR-RFLP in Sulaimani, Iraq". test, 14, 2, 2022, 115-126. doi: 10.21608/eajbse.2022.269215
Mahmood, D., Osman, Y., Nidhamaddin, S., Ahmadissa, S., Rahim, A., Salih, M., Hamasoor, T. (2022). 'Identification of Candida Species Isolated from Oral Candidiasis in Cancer Patients using PCR-RFLP in Sulaimani, Iraq', test, 14(2), pp. 115-126. doi: 10.21608/eajbse.2022.269215
Mahmood, D., Osman, Y., Nidhamaddin, S., Ahmadissa, S., Rahim, A., Salih, M., Hamasoor, T. Identification of Candida Species Isolated from Oral Candidiasis in Cancer Patients using PCR-RFLP in Sulaimani, Iraq. test, 2022; 14(2): 115-126. doi: 10.21608/eajbse.2022.269215

Identification of Candida Species Isolated from Oral Candidiasis in Cancer Patients using PCR-RFLP in Sulaimani, Iraq

Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology
Volume 14, Issue 2, December 2022, Pages 115-126    PDF (652.37 K)
Document Type: Original Article
DOI: 10.21608/eajbse.2022.269215
Authors
Diman M. Mahmood1; Yad N. Osman2; Sara J. Nidhamaddin2; Solhan M. Ahmadissa1; Alaa A. Rahim3; Mariwan A. Salih4; Taib A. Hamasoor5
1Medical Laboratory Department, College of Health and Medical Technology, Sulaimani Polytechnic University, Sulaimani, Iraq.
2Medical Oncologist, Department of Medical Oncology, Hiwa Cancer Hospital, Sulaimani, Iraq.
3Medical Laboratory Analysis, Cihan University-Sulaimanya, Slemani, Iraq.
4Medical Laboratory Department, College of Health and Medical Technology, Sulaimani Polytechnic University, Sulaimani, Iraq - Medical Laboratory Analysis, Cihan University-Sulaimanya, Slemani, Iraq.
5Medical Laboratory Department, College of Health and Medical Technology, Sulaimani Polytechnic University, Sulaimani, Iraq. - Medical Laboratory Analysis, Cihan University-Sulaimanya, Slemani, Iraq.
Abstract
Candida species causes oral candidiasis in immunosuppressed individuals such as cancer patients. This study aimed to determine the rate of candida infections in cancer patients (n= 150) and to identify different species of candida using Polymerase Chain Reaction-restriction fragment polymerase length polymorphism (PCR-RFLP), and to find the effect of candidiasis on the PH of the saliva in cancer patients (n=50) and controls (n=50) (Healthy individuals). The rate of candidiasis was different in patients with different types of cancer.  A higher percentage of oral Candidiasis was observed in carcinoma (54 %), followed by leukemia patients (17.3 %), sarcoma type (15.3%), and lymphoma (13.33%). The highest prevalence of candidiasis was found in the old age group, 40-60years Candida albicans was the most common recorded species,70% in comparison to non- albicans species, followed by other species including: Candida glabrata (12%), Candida kefyr (6.7%), Candida tropicalis (5.3%), Candida krusei (3.3%), and Candida dubliniensis (2.7 %). The pH of saliva was more acidic in cancer patients who have candidiasis in comparison to control individuals (P-value 0.001). In conclusion, this study found that the rate of candidiasis is high among cancer patients and Candida albican is the most common species recorded in cancer patients.
Keywords
Oral Candidiasis; Cancer patients; PCR-RFLP; Species of candida
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