HPLC fingerprint profile, in-vitro cytotoxicity and anti-herpes simplex virus activity of methanol extract from Strophanthus hispidus DC (Stem bark). | ||
| Microbes and Infectious Diseases | ||
| Article 39, Volume 5, Issue 1, February 2024, Pages 379-388 PDF (1.02 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2022.171059.1409 | ||
| Authors | ||
| Arnold Mulula* 1; Abdeldjalil Bouzina2; Hugues Bisi Mambu3; Joséphine Kankolongo Ntumba1; Joachim Nsomue1; Milka Ndaya Tshingamb4; K.T. Kashishi1; Ahmed Zaki5; Taba Kalulu1 | ||
| 1Department of Chemistry, Faculty of Sciences and technologies, University of Kinshasa (Democratic Republic of Congo) | ||
| 2Department of Botany and Microbiology, Faculty of Sciences, Cairo University, Egypt. | ||
| 3Cliniques Universitaires de Kinshasa, University of Kinshasa, Congo. | ||
| 4Department of Chemistry, Faculty of Sciences and technologies, University of Kinshasa (Democratic Republic of Congo) Institut National de Recherches Biomédicales (INRB) Democratic Republic of Congo | ||
| 5Department of Chemistry, Faculty of Sciences, Ainshams University, Egypt. | ||
| Abstract | ||
| Herpes simplex virus (HSV) infection is quite common in adults and neonates and is associated with oral mucocutaneous lesions and/or genital infections. Therefore, the aim of this present study was to determine the in-vitro cytotoxicity and anti-herpes simplex virus (1 and 2) activity of methanol extract of Strophanthus hispidus (S. hispidus ) DC (Stem bark) and his HPLC fingerprint profile. The cytotoxicity test on Vero cells was performed by MTT assay and various concentrations of extract less than the maximum nontoxic concentration were used against Vero cells for anti-HSV-1 and ant-HSV-2 effects by plaque assay. The CC50 values of methanolic extracts of S. hispidus (stem bark) and acyclovir against Vero cells were 94.46 µg/mL and 118.78 µg/mL, respectively. Whereas the EC50 and selectivity index values of this extract against herpes simplex virus 1 and herpes simplex virus 2 strains were 36.60 µg/mL (SI=2.58) and 39.47 µg/mL (SI=2.39), respectively. HPLC finger-printing of this extract was developed and showed seven major compounds including ascorbic acid, quercetin, resorcinol and gallic acid represented in large amount. In conclusion, the methanolic extract of Strophanthus hispidus DC(stem bark) has good antiherpetic activity against HSV- 1 and HSV-2 but it is also highly toxic. Therefore, it will be important to separate toxic molecules from those responsible of the antiherpetic activity by bio guided fractionation procedures. | ||
| Keywords | ||
| Strophanthus hispidus DC; Cytotoxicity; anti-Herpes Simplex Virus type 1 and 2; antiviral activity; HPLC | ||
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