Hussein, S., Abo zaid, O., Abdelmaksoud, H., Ismael, T., Al lawaty, G. (2022). Hesperidin and Rosemary extract alleviates apoptosis and alterations of DNA methyltransferase and targeting microRNA in a rat model of diabetic cardiomyopathy. test, 42(2), 31-36. doi: 10.21608/bvmj.2022.148089.1544
Samy Ali Hussein; omayma ragab Abo zaid; Hussein F Abdelmaksoud; Taheya F Ismael; Ghada Fathy Al lawaty. "Hesperidin and Rosemary extract alleviates apoptosis and alterations of DNA methyltransferase and targeting microRNA in a rat model of diabetic cardiomyopathy". test, 42, 2, 2022, 31-36. doi: 10.21608/bvmj.2022.148089.1544
Hussein, S., Abo zaid, O., Abdelmaksoud, H., Ismael, T., Al lawaty, G. (2022). 'Hesperidin and Rosemary extract alleviates apoptosis and alterations of DNA methyltransferase and targeting microRNA in a rat model of diabetic cardiomyopathy', test, 42(2), pp. 31-36. doi: 10.21608/bvmj.2022.148089.1544
Hussein, S., Abo zaid, O., Abdelmaksoud, H., Ismael, T., Al lawaty, G. Hesperidin and Rosemary extract alleviates apoptosis and alterations of DNA methyltransferase and targeting microRNA in a rat model of diabetic cardiomyopathy. test, 2022; 42(2): 31-36. doi: 10.21608/bvmj.2022.148089.1544

Hesperidin and Rosemary extract alleviates apoptosis and alterations of DNA methyltransferase and targeting microRNA in a rat model of diabetic cardiomyopathy

Benha Veterinary Medical Journal
Volume 42, Issue 2, September 2022, Pages 31-36    PDF (1.07 M)
Document Type: Original Article
DOI: 10.21608/bvmj.2022.148089.1544
Authors
Samy Ali Hussein1; omayma ragab Abo zaid2; Hussein F Abdelmaksoud3; Taheya F Ismael4; Ghada Fathy Al lawaty* 3
1Department of Biochemistry, Faculty of Vet. Med., Benha University, Egypt.
2Biochemistry veterinary medicine Banha university
3Biochemistry- veterinary medicine- benha University-Egypt
4Nutrition &Clinical Nutrition- veterinary medicine- benha University-Egypt
Abstract
Diabetic cardiomyopathy (DCM) is a complication of diabetes results in high mortality due to its oxidative stress. The present study was designed to investigate the effect of rosemary extract and Hesperidin on hyperglycemia-induced oxidative stress, inflammation and cardiac injury in experimentally induced DCM in rats. Fifty rats were divided into five groups. Group Ι: rats fed on normal diet. Group Π: rats received a single dose of streptozotocin at a dose of (50 mg /kg body wt.). Group III: Rats treated with insulin 2 U/rat per day. Group IV: rats treated orally with Rosemary extract at a dose of (200 mg/kg body weight/day). Group V: rats treated with Hesperidin orally at a dose of (100 mg/kg body weight/day). Blood samples and cardiac tissue specimen were collected at the end of the experiment. The obtained results showed a marked increase in blood glucose concentration and upregulation in Endothelin-1(ET-1), Hypoxia inducing factor-1 α (HIF-1α), miR-29 and miRNA130b-3p gene expressions with hypermethylation in global DNA methyltransferase (DNMT) in cardiac tissue of DCM-induced group. Treatment with insulin, rosemary extract and hesperidin exhibited a significant decrease in blood glucose concentration and downregulation in Endothelin-1, miR-29 and miRNA130b-3p expressions with hypomethylation in DNMT, while, HIF-1α showed a significant upregulation as compared to DCM non treated group. In conclusion, these results indicated that rosemary extract and hesperidin may have great therapeutic potential in the treatment of DCM.
Keywords
Diabetic cardiomyopathy; rosemary extract; hesperidin; Endothelin-1; DNA methyltransferase
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