Using Molecular Tools to Compare Various Concentration Protocols Prior to Detecting SARS-Cov-2 RNA in Wastewater | ||
| Egyptian Journal of Chemistry | ||
| Volume 65, Issue 131, December 2022, Pages 1241-1248 PDF (324.94 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2022.165190.7030 | ||
| Authors | ||
| Dina Nadeem Abd-Elshafy* 1; Rola Nadeem2; Mahmoud Bahgat3 | ||
| 1Water pollution research department, institute of environmental research and climate change, National research center,giza,Egypt | ||
| 2Therapeutic chemistry department, Institute of pharmaceutical and drug industry research, National research center, Giza, Egypt | ||
| 3Therapeutic Chemistry Department, National Research Centre (NRC), 33 EL Bohouth St. (former El Tahrir St.), | ||
| Abstract | ||
| Background: Reports showed presence of SARS-CoV-2 genetic material in wastewater. Wastewater concentration methods are optimized for detection of non-enveloped viruses so need to be adopted for enveloped viruses and their genetic material. Methods: Conventional (cRT-PCR) and quantitative real time RT-PCR (qRT-PCR) were used as readouts to compare 4 water concentration methods namely, A) filtration on negatively charged membrane followed by extracting RNA from it, B) adsorbtion-elution method, C) flocculation with skimmed milk and D) polyethylene glycol precipitation, to detect SARS-CoV-2 RNA and 229E human coronavirus (229E-HCoV) as a model for spike-containing enveloped virus from fresh and wastewater. Results: On using cRT-PCR: recovery rate of SARS-CoV-2 RNA was better using method A then B for fresh water and method B then D for wastewater. 229E-HCoV recovery from fresh water was better using method C then A and methods B then D for wastewater. On using qRT-PCR, both methods A and B were better for SARS-CoV-2 RNA recovery from both fresh and wastewater. For the 229E-HCoV methods A was the most efficient for fresh water and method B for wastewater. Conclusion: Method B is recommended for SARS-CoV-2 RNA or whole 229E-HCoV recovery from wastewater. | ||
| Keywords | ||
| SARS-CoV-2; Human corona virus 229E; molecular tools; wastewater; Concentration methods | ||
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