Expression profiles of catalase gene in common carp exposed to ammonia | ||
| SVU-International Journal of Veterinary Sciences | ||
| Volume 5, Issue 4 - Serial Number 14, December 2022, Pages 114-124 PDF (928.46 K) | ||
| Document Type: Research article | ||
| DOI: 10.21608/svu.2022.155871.1223 | ||
| Authors | ||
| Mahmoud Nasr1; Ahmad A.A. Elkamel* 2; Mohammed Youssef3; Walaa F.A. Emeish1 | ||
| 1Department of Fish Diseases, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt | ||
| 2Department of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, Assiut University | ||
| 3Department of Animal physiology, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt | ||
| Abstract | ||
| Ammonia is one of the most harmful water quality parameters restricting the growth and survival of aquatic living organisms. As a result, fish must adapt to this stressor by modifying physiological processes that are governed by gene expression regulation. The aim of the present study was to investigate the expression profiles of antioxidant related gene, catalase (CAT) in common carp (Cyprinus carpio) after exposure to 0.7 mg/l of unionized ammonia (UIA) in water. The relative gene expression was measured in liver, gills, and brain tissues at four time points (12h, 2d, 4d, and 7d of exposure). The expression level of CAT gene in the liver and brain peaked after 7d of ammonia exposure by 13.3 and 5.2folds, respectively, but in gills it upregulated only after 2d (2.7folds) and downregulated at the other time points. This study proves that exposure to ammonia affects the antioxidant status of common carp as indicated by the altered levels of expression of the CAT gene. | ||
| Keywords | ||
| common carp; ammonia; gene expression; antioxidant-related genes; CAT | ||
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