CONJUGATION OF IMMUNOGLOBULINS PREPARED AGAINST INFECTIOUS BURSAL DISEASE VIRUS IN DIFFERENT HOSTS WITH FLUORESCINE ISOTHIOCYANATE | ||
| Egyptian Journal of Agricultural Research | ||
| Article 30, Volume 82, Issue 4, December 2004, Pages 1881-1891 PDF (2.35 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejar.2004.275755 | ||
| Authors | ||
| NABEL A. ABD ELWANIS; MOHAMED H. KHODEIR | ||
| Veterinary Serum and Vaccine Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt | ||
| Abstract | ||
| Three different immunoglobulins were prepared against infectious bursal disease virus (IBDV) in different hosts (chicken, ducks and rabbits) and conjugated with fluorescine isothiocyanate (FITC).The efficacy of these preparations was evaluated through the scoring of IBDV concentration in different sites of infected specific pathogen free (SPF) embryonated chicken eggs with different strains of the virus (Bursa-Vac., D-78 and 228E) using the direct fluorescent antibody technique (FAT) in a comparison with the virus titration test. It was found that the prepared conjugates were able to detect the IBDV in the different sites of infected eggs when they were diluted up to 1: 10' giving strong; moderate or weak positive FAT according to the virus concentration in the tested site. There were no differences in the experimental results among the different used viral strains, so, the prepared IBDV immunoglobulins conjugated with FFFC provide local products that facilitate and enhance a rapid and accurate diagnosis of MD the thing which is required to achieve a successful control. | ||
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