First report of Candidatus Phytoplasma Asteris Infecting Lily in Egypt | ||
| Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||
| Volume 14, Issue 2, December 2022, Pages 381-388 PDF (657.3 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsc.2022.276644 | ||
| Authors | ||
| Aly M. Abdel-Salam1; Nermeen Taha Shanan2; Doaa Z. Soliman3; M. A. Ahmed3 | ||
| 1Plant Pathology Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt | ||
| 2Horticulture Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt. | ||
| 3Plant Pathology Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt. | ||
| Abstract | ||
| During the inspection of imported grown Lily (Lilium spp.) plants (Acapulco cultivar), symptoms of Candidatus Phytoplasma asteris (Ca. P. astris) infestation was observed in the experimental fields of the Faculty of Agriculture, Cairo University, Giza governorate. The observed disease symptoms included stunting, scorched and leaf discoloration, flowering bud failure, and reduced quality of bulbs. Nested polymerase chain reaction (PCR) was the key technique employed in this study. Four pairs of primers were used in PCR analysis. In the first amplification, a universal primer pair P1/P7 was used to amplify a 1.8 kbp DNA fragment of the rRNA operon. In the second nested amplification, the universal primer pairs R16F2n/R16R2 (F2n/R2) and R16F0/R16R1 (F0/R1) and the specific P1/Ayint were used to detect 1.2, 1.44, and 1.5 kbp, respectively, from aster yellows group phytoplasma infecting plants. Containment of phytoplasma infestation in lily was obtained through a periodical spray of infected lily plants with tetracycline hydrochloride antibiotic. Lily plants are vegetatively propagated. This allows the spread of this phytoplasma to several economic crops. | ||
| Keywords | ||
| Lily; Aster yellows phytoplasma; nested PCR; tetracycline hydrochloride | ||
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