MicroRNA-146a and MiR-155 Expression in Toxoplasmosis Patients with Rheumatoid Arthritis | ||
| The Egyptian Journal of Hospital Medicine | ||
| Article 241, Volume 89, Issue 2, October 2022, Pages 7714-7718 PDF (379.99 K) | ||
| DOI: 10.21608/ejhm.2022.277125 | ||
| Authors | ||
| Huda Dhaher Al-Marsomy* 1; Zainab Abed Muhsin AL-Haboobi2; Qudus Wamidh Jamal1 | ||
| 11Al-Nahtrain, College of Medicine, Department of Microbiology, Baghdad, Iraq | ||
| 22AL-Furat Al-Awsat Technical University/ Karbala Technical Institute, Karbala, Iraq | ||
| Abstract | ||
| Introduction: Toxoplasma gondii is a protozoan parasite that lives intracellular and has been linked to autoimmune disease. It also can modulate immune responses to varying degrees. Rheumatoid arthritis, sometimes known simply as RA, is a prevalent autoimmune disease that is distinguished by its multiple pathogenic pathways, which ultimately lead to persistent inflammation and damage to the joints. MicroRNAs play a crucial role in immune response regulation and can be used as novel biomarkers to improve diagnosis, treatment, and disease states. Objective: The purpose of this study was to investigate the levels of expression of the microRNAs miR-146a and miR-155 as potential biomarkers for the diagnosis of rheumatoid arthritis (RA) in individuals who were infected with toxoplasmosis. Patients and Methods: The study was conducted on 50 Rheumatoid Arthritis patients positive for toxoplasmosis as diagnosed by ELISA (divided into two groups according to treatment response) and 50 age and sex-matched healthy subjects as a control group. Purified RNA was done, then real-time PCR was carried out using specific primers for MicroRNA-146a and MiR-155. Results: A significant up-regulation in both Micro-RNA in the studied groups when compared with the control group. A high level of miR-146 expression was associated with low expression for miR-155 observed in the negative response group for treatment when compared with the positive response group. Conclusion: the miR-146 and mir-155 could be used as biomarkers for host response during the biological treatment. | ||
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