Urinary Organic Acids as Non-Invasive Biomarkers of Egyptian | ||
| The Egyptian Journal of Hospital Medicine | ||
| Article 253, Volume 89, Issue 2, October 2022, Pages 7786-7791 PDF (1002.51 K) | ||
| DOI: 10.21608/ejhm.2022.277144 | ||
| Authors | ||
| Ebtihal Mohammed* 1; Samar E. Ghanim1; Ashraf El Fert1; Menan El said Salem2; Hala El- Said3 | ||
| 1Departments of 1Biochemistry and Molecular Diagnostics | ||
| 22Pediatric Hepatology, National Liver Institute, Menoufia University, Egypt | ||
| 3El- Said | ||
| Abstract | ||
| Background: There are significant global efforts being made to eradicate the hepatitis C virus (HCV). The outcome of HCV treatment has altered significantly as a result of the use of direct-acting antivirals (DAAs). The oral drugs sofosbuvir (SOF) and ledipasvir (LED) are recommended by the World Health Organization for the treatment of HCV in adolescents older than or equivalent to 12 years old. To the best of our knowledge, the impact of HCV clearance by DDAs on the metabolic pathways in the liver has not been examined in any metabolomics research to date. Objective: This study was aimed to evaluate urinary organic acids before and after viral eradication by DDAs which reflect the metabolic status of the liver. Patients and Methods: This prospective study included 50 pediatric cases of chronic hepatitis C (CHC) genotype 4 who fulfilled the inclusion and exclusion criteria in addition to 50 healthy controls. All participants underwent routine laboratory investigations, HCV polymerase chain-reaction (PCR) test and urinary organic acids using gas chromatography mass spectrometry (GC/MS) before and at the end of treatment. Results: The study indicated that liver enzymes were decreased significantly after viral clearance (All P > 0.05), Urinary 2 keto-glutarate (P1<0.001), hippuric acid (P1<0.001), acetic acid (P1<0.001), phenylacetic acid (P1<0.002), palmitic acid(P1<0.007) and stearic acid (P1<0.027) were decreased significantly after treatment while L-fucose (P1<0.001), D-xylose (P1<0.001), arabinose (P1<0.002), L-rhamnose (P1<0.001), and D-ribose (P1<0.001) were increased significantly after treatment. Urinary organic acids profile after treatment became nearer to healthy control. Conclusion: Viral clearance by DDAs has improved the metabolic functions of the liver so it is an effective treatment.Metabolomics can be used as non-invasive biomarker to assess the liver pathological and physiological state and to assess the efficacy of new drugs. | ||
|
Statistics Article View: 172 PDF Download: 302 |
||
