Molecular Research of The Difference between Chromosome and Plasmid at Harboring Some Virulence and Antibiotic Resistance Genes in P. Mirabilis | ||
| The Egyptian Journal of Hospital Medicine | ||
| Article 277, Volume 89, Issue 2, October 2022, Pages 7950-7957 PDF (658.32 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejhm.2022.277399 | ||
| Authors | ||
| Huda Qasim Owaied; Sanaa Ghali Jabur | ||
| Abstract | ||
| Background: Proteus spp < em>. is gram negative bacteria belonging to the Enterobacteriaceae family. P. mirabilis is the most commonly isolated species from clinical samples. Objective: The present study aimed to investigate and compare the prevalence of virulence genes (hpmA, hpmB) and the antibiotic resistant genes (blaCTX-M and blaKPC) on both chromosome and plasmid in Proteus mirabilis isolates. Material and methods: A total of 487 samples were collected from different clinical sources. Samples were obtained for patients of different ages were involved of both sexes. The samples were collected from hospitals and private laboratories in Thi-Qar province, Iraq during a period from November 2021 to Abril 2022. A total of the isolates were diagnosed by different laboratory and molecular methods. Results: The prevalence of P.mirabilis was 8.6 % among collected samples. The findings of virulence genes (hpmA and hpmB) indicated that 45% of isolates were positive for hpmA gene on chromosome and 33.3% on plasmid, while hpmB gene rate was 30.9% on chromosome and 16.7% on plasmid. On the other hand the antibiotic resistance genes (blaCTX-M and blaKPC) test results showed that the blaCTX-M gene was absent on the chromosome with a very high frequency on the plasmid (95.2%). While the blaKPCappeared in a low rate on the chromosome (38%) but it has been risen on the plasmid (95.2%). Conclusion: The examined virulence genes (hpmA, hpmB) were found mostly on the chromosome while the antibiotic resistance genes (blaCTX-M, blaKPC) found mostly on the plasmid. | ||
| Keywords | ||
| P. mirabilis; virulence genes; hemolysins; antibiotic resistance genes; University of Thi-Qar; Iraq | ||
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