STUDIES ON THE EFFICACY OF GARLIC EXTRACT ON CRYPTOSPORIDIOSIS IN EXPERIMENTALLY INFECTED MICE | ||
| Egyptian Journal of Agricultural Research | ||
| Article 28, Volume 81, Issue 2, July 2003, Pages 793-803 PDF (3.55 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejar.2003.278037 | ||
| Author | ||
| AHMED A. WAHBA | ||
| Animal Health Research Institute, Agricultural Research Centre, Dokki, Giza, Egypt | ||
| Abstract | ||
| The present work was conducted to detect the efficacy of garlic extract on Cryptosporidium parvum in experimentally infected mice. Thirty six Swiss mice aging 2 — 3 weeks were divided into 3 groups, each of 12 mice. Group I was the infected treated, group II was the infected non-treated control and group Ill was the non-infected non-treated control one. The mice in the infected groups were given 106 C. parvum oocysts. Garlic extract was given orally to mice of the treated group at a dose of I ml / 100 g body weight. Faecal smears from mice of each group were examined every 2 days after staining by modified Zieht Neelsen staining technique and the oocysts were counted in 100 randomly selected field at X 1000 magnification. The results showed that, there was a significant difference in the mean values of oocysts shedding between the infected treated group I and the infected non-treated control group II from the 7th day posttreatment (d.p.t.) (mean ± SD; 25.17 ± 5.56 and 40.33 t 16.72 respectively) until the termination of the experiment on the 25th d.p.t. (mean ± SD) 0.94 ± 1.43 and 55.75 ± 19.79 respectively). The therapeutic efficacy of garlic extract on cryptosporidiosis in experimentally infected mice was 58.3%. At the termination of the experiment on the 25th d.p.t., the mice of the three groups were sacrificed and the ilea were collected for histopathological examination. The villi of Ilea in the infected-treated mice in group I showed no pathological changes and regained their normal appearance. They showed neither atrophy nor desquamation of villi when compared to ilea of infected-non treated control mice. At the end of experiment, some infected-treated mice (5/12) belonging to group I showed some degrees of oocysts count in faeces, but didn't show neither atrophy nor desquamation of | ||
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