THE IN VITRO ACTIVITY OF CHITOSAN NANOPARTICLES VERSUS METRONIDAZOLE AGAINST BLASTOCYSTIS HOMINIS | ||
| Journal of the Egyptian Society of Parasitology | ||
| Volume 52, Issue 3, December 2022, Pages 501-508 PDF (982.26 K) | ||
| DOI: 10.21608/jesp.2022.278372 | ||
| Authors | ||
| WAFAA M. ZAKI1; HEDAYAT A. SALEM2; AYMEN M. MADKHALY3; SALWA F. OSHIBA4 | ||
| 1Department of Medical Parasitology, Faculty of Medicine, Suez Canal University, Ismalia | ||
| 2Department of Clinical Pathology, Faculty of Medicine, Cairo University, Egypt | ||
| 3Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Jazan University, Kingdom of Saudi Arabia | ||
| 4Department of Parasitology, Faculty of Medicine, Menoufia University, Egypt | ||
| Abstract | ||
| Blastocystis spp. is a protozoan parasite with controversial clinical significance and consensus for treatment. Metronidazole was considered the main line of treatment, but currently wide range of efficacy rate, drug resistance and many undesirable side effects motivated the search for a novel effective safe therapy. Chitosan nanoparticles have offered a new pharmacological tool for the treatment of many parasites especially protozoan parasites. In vitro antiprotozoal activity of Chitosan Nanoparticles (Cs NPs) on Blastocystis spp. was assessed through challenging with graded concentrations (20, 40, & 50μg/ml) compared to MTZ (100μg/ml), parasite count and viability was assessed after 24, 48 & 72hr. of incubation. Chitosan nanoparticles offered a remarkable inhibition activity on blastocystis growth and viability, which were proportional to its concentration and exposure duration, Cs NPs 20μg/mL gave a significant growth inhibition percentage after 24hr & 48hr (56.4% & 50.5%) respectively, regarding Cs NPs 40μg/ml, it was (73.1% & 86.9%) respectively. While highly significant decrease in mean parasitic count 99.2% was occurred when 50μg/ml Cs NPs was used for 72hr compared to 89.8% MTZ for the same duration. Meanwhile, metronidazole (100μg/ml) was effective in treatment of blastocystis more than Cs NP 20μg/ml, but it was less effective than Cs NP 40μg/mL and Cs NP 50μg/ml for all duration time. | ||
| Keywords | ||
| Blastocystis spp; in vitro; Cs NPs; Metronidazole | ||
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