Frequency of Metallo-Β-Lactamase Among Pseudomonas aeruginosa Isolated from patients In Intensive Care Units and Operating Rooms | ||
| Egyptian Academic Journal of Biological Sciences, G. Microbiology | ||
| Volume 15, Issue 1, June 2023, Pages 1-11 PDF (633.53 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsg.2023.280058 | ||
| Authors | ||
| Wafaa M. A. Abdulrahaman11; Eldaw B. S. Mohamed2; Fatimah M. S. Saidi3; Mogahid M. Elhassan4 | ||
| 1Microbiology Dept. College of Medicine Batterjee Medical College. -Department of Medical Microbiology, College of Medical Laboratory Science, Kordofan University, Sudan. | ||
| 2Department of Medical Microbiology, College of Medical Laboratory Science, Kordofan University, Sudan. | ||
| 3Physical therapy Dept. Batterjee Medical College | ||
| 4Nahrain College, Khartoum, Sudan. -Department of Medical Microbiology, College of Medical Laboratory Science, Sudan University of Science and Technology, Sudan | ||
| Abstract | ||
| Background: P.aeruginosa infection is one of the major health problems in the world. It’s an opportunistic human pathogen that causes serious problems. Usually resistant to several antibiotics, show a particular ability to spread in hospitals. In recent times, Metallo-β-lactamase resistance in this bacterium has imposed some difficulties in treating bacterial infections. This study was a qualitative study, aimed to detect Metallo-β-Lactamase in Carbapenems-resistant P. aeruginosa isolated from inpatients admitted to Intensive Care Unit and Operating Room in different hospitals in Jeddah city, KSA during the period from March 2019 to September 2022.Methods: In this study, a total of (234) were cultured and identified using API 12A/12E. All isolates were subjected to antimicrobial susceptibility testing using Kirby Bauer method, for selected imipenem and meropenem. Results: Out of 234 specimens 154 Pseudomonas aeruginosa were isolated and identified, with different ratios (males, 62.3: females, 37.7), which occurred highest in the adult age group. The highest frequency of isolate 152 (98.7%) was in the intensive care unit while the lowest frequency of isolate 2 (1.3%) was in the operating room. (9%), (5.8%), (10.38%) and (1.2%) were found resistant to imipenem, meropenem, ciprofloxacin and amikacin respectively.Conclusion: Carbapenems have great bactericidal activity against Ps. aeruginosa, while, this notorious pathogen acquisition resistance against these drugs and limited treatment options. Our isolated strains showed a low rate of resistance (9%) and (5.8%) against imipenem and meropenem respectively. Aminoglycoside is crucial for the treatment of various Ps. aeruginosa infections. However, our study showed that (1.2%) of Ps. aeruginosa strains were resistances to amikacin and Ciprofloxacin has been extensively used to treat wide a variety of Ps. aeruginosa infections. While Ps. aeruginosa rapidly acquired resistance to ciprofloxacin that creating a therapeutic challenge. In this study, 10.38% of Ps. aeruginosa was found resistant to Ciprofloxacin. We recommended further studies and a regular monitoring system for the early detection of MBL-producing organisms. | ||
| Keywords | ||
| Pseudomonas aeruginosa; Metallo-Β-Lactamase; intensive care units | ||
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