Zaki, A., Amer, W., Abo Elezz, A., Mohamed, W. (2019). Study of Some Enteropathogens Causing Acute Diarrhea in Infants and Children Less than 5 Years Old. test, 28(2), 145-151. doi: 10.21608/ejmm.2019.282758
Amira E. Zaki; Wesam H. Amer; Ahmed A. Abo Elezz; Waseela M. Mohamed. "Study of Some Enteropathogens Causing Acute Diarrhea in Infants and Children Less than 5 Years Old". test, 28, 2, 2019, 145-151. doi: 10.21608/ejmm.2019.282758
Zaki, A., Amer, W., Abo Elezz, A., Mohamed, W. (2019). 'Study of Some Enteropathogens Causing Acute Diarrhea in Infants and Children Less than 5 Years Old', test, 28(2), pp. 145-151. doi: 10.21608/ejmm.2019.282758
Zaki, A., Amer, W., Abo Elezz, A., Mohamed, W. Study of Some Enteropathogens Causing Acute Diarrhea in Infants and Children Less than 5 Years Old. test, 2019; 28(2): 145-151. doi: 10.21608/ejmm.2019.282758

Study of Some Enteropathogens Causing Acute Diarrhea in Infants and Children Less than 5 Years Old

Egyptian Journal of Medical Microbiology
Volume 28, Issue 2, April 2019, Pages 145-151    PDF (295.29 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2019.282758
Authors
Amira E. Zaki* 1; Wesam H. Amer1; Ahmed A. Abo Elezz2; Waseela M. Mohamed1
1Medical Microbiology &Immunology Department, Faculty of Medicine, Tanta University
2Pediatrics Department, Faculty of Medicine , Tanta University
Abstract
Background: Diarrheal disorders are a major health issue in pediatrics all over the world. Objectives: To isolate enteropathogens causing diarrhea and determine antibiotic sensitivity pattern. Methodology: 120 stool samples underwent the following procedures; macroscopic and microscopic examination, preparation of wet smear, detection of fecal leucocytes ,carbol-fuchsin staining for detection of Campylobacter, Rota virus detection by immunochromatography, culture of samples on Mackongey agar, XLD, API for lactose non fermenting colonies, Identification of EPEC by polyvalent antisera , O157:H7 E.coli detection using Sorbitol Mackoncey and the isolates obtained were tested for antimicrobial sensitivity. Results: The detected enteropathogens were EPEC (46.6%), Rota Virus (20.8%), Salmonella (14.2%), Shigella (1.7%), K.oxytoca (8.3%)and Pseudomonas (4.2%).The antibiotic resistance pattern was ESBL; EPEC (53%), Salmonella (47%). ESBL and Ampc; EPEC (65%), Shigella (5%) and K.oxytoca (25%). Ampc resistance was EPEC while all Pseudomonas isolates were wild. Conclusions: Rehydration therapy, Rota virus vaccination and antibiotic policy are essential for management of diarrheal cases.
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