MSCRAMMs Encoding Genes Expression in MRSA Isolated from Children with Cleft Lip and Palate and its Relation to the Phenotypic Ability of Biofilm Formation | ||
| Egyptian Journal of Medical Microbiology | ||
| Volume 28, Issue 2, April 2019, Pages 153-156 PDF (325.8 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2019.282759 | ||
| Authors | ||
| Hanan S. Abdel Khalek* 1; Mona O. Ramadan1; Amir F. Abedlhamid2 | ||
| 1Medical Microbiology and Immunology Department Faculty of Medicine Tanta University | ||
| 2General Surgery Department, Tanta University Hospital | ||
| Abstract | ||
| Objectives: Our study aimed to detect the correlation between the MSCRAMMs gene expression and the ability of MRSA to produce biofilm phenotypically. Methodology: We studied staph aureus isolated from cleft lip and cleft palate of congenital mal or deformed children. Total of 100 isolates were collected from cleft lip and palate of children from plastic surgery department of Tanta University Hospital during a period of 11 months. All samples were subjected to phenotypic methods then biofilm forming strains were assessed for genes by multiplex PCR. Results: 85/100 of samples were stap. Aureus 28/85 were MRSA .Phenotypic aspect showed that 56% of strains were high biofilm producers while 30% were intermediate and 14% were low producer. fib gene was the most prevalent gene among tested strains producing biofilm. Conclusion: MRSA harbor MSCRAMMs encoding gene family, There are several genes icaA&B&D & eno &ebps anf fib are the targeted in our study. There is a strong correlation between ability of biofilm formation and its genotypic control. | ||
| Keywords | ||
| Biofilm; Gene expression; MRSA | ||
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