Phenotypic and Molecular Characterization of Aminoglycoside Resistance in Clinical Escherichia coli Isolates from Patients at Menoufia University Hospitals | ||
| Egyptian Journal of Medical Microbiology | ||
| Volume 28, Issue 3, July 2019, Pages 25-32 PDF (584.86 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2019.282956 | ||
| Authors | ||
| Mabrouk M Ghonaim; Rasha G Mostafa; Shymaa S Alkady* | ||
| Department of Microbiology and Immunology, Faculty of Medicine, Menoufia University, Egypt | ||
| Abstract | ||
| Background: E. coli may develop different complex mechanisms of resistance to various classes of antibiotics. Objectives: To assess the prevalence of E. coli in Menoufia University Hospitals, to evaluate its antibiotic resistance profile and to detect the presence of aminoglycoside resistance genes [aac(3)IIa and aac(6)Ib] by real-time polymerase chain reaction (PCR). Methodology: A total of 200 clinical samples were collected from patients in Menoufia University Hospitals. E. coli was identified by standard microbiological methods. E. coli isolates were tested by the modified Kirby-Bauer disk diffusion method, and for extended-spectrum β-lactamases production. Aac(3)IIa and aac(6)Ib genes were detected by real-time PCR. Results: E. coli forms 25% of the isolated bacterial strains. E. coli isolates showed the highest resistance to pipracillin and amoxicillin-clavulanate (96%). On the other hand, carbapenemes had the least resistance rate (30%) followed by amikacin (44%), gentamicin (60%), tobramycin (60%) and streptomycin (64%). ESβL production was found among 70% of the isolates by the screening and 50% by the confirmatory methods. Real-time PCR showed that 84% of the isolates contain aac(3)IIa, 52% contain aac(6)Ib and 46% contain both genes. Conclusion: E. coli showed resistance to different classes of antibiotics including aminoglycosides with high prevalence of aac(3)IIa and aac(6)Ib genes. Therefore, infection control measures and rationalized use of antibiotics are mandatory. | ||
| Keywords | ||
| Aminoglycoside resistance; Escherichia coli; Phenotypic; Molecular Characterization | ||
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