Osman, S., Jones, S., Zewail, M., Rabie, A., Shalaby, A., Howl, J. (2019). Microwave Assisted Peptide Synthesis of Some Rationally Designed Cell Penetrating Peptides from C-Kit Receptor. test, 62(8), 1377-1389. doi: 10.21608/ejchem.2019.7119.1611
Shaimaa Osman; Sarah Jones; Mohamed Zewail; Abdelgawad Rabie; Ahmed Shalaby; John Howl. "Microwave Assisted Peptide Synthesis of Some Rationally Designed Cell Penetrating Peptides from C-Kit Receptor". test, 62, 8, 2019, 1377-1389. doi: 10.21608/ejchem.2019.7119.1611
Osman, S., Jones, S., Zewail, M., Rabie, A., Shalaby, A., Howl, J. (2019). 'Microwave Assisted Peptide Synthesis of Some Rationally Designed Cell Penetrating Peptides from C-Kit Receptor', test, 62(8), pp. 1377-1389. doi: 10.21608/ejchem.2019.7119.1611
Osman, S., Jones, S., Zewail, M., Rabie, A., Shalaby, A., Howl, J. Microwave Assisted Peptide Synthesis of Some Rationally Designed Cell Penetrating Peptides from C-Kit Receptor. test, 2019; 62(8): 1377-1389. doi: 10.21608/ejchem.2019.7119.1611

Microwave Assisted Peptide Synthesis of Some Rationally Designed Cell Penetrating Peptides from C-Kit Receptor

Egyptian Journal of Chemistry
Article 3, Volume 62, Issue 8, August 2019, Pages 1377-1389    PDF (2.73 M)
Document Type: Original Article
DOI: 10.21608/ejchem.2019.7119.1611
Authors
Shaimaa Osman* 1; Sarah Jones2; Mohamed Zewail3; Abdelgawad Rabie4; Ahmed Shalaby5; John Howl6
1Peptide Chemistry Department, Chemical Industries Research Division, National Research Centre, Cairo, Egypt.
2Research Institute in Healthcare Science, School of Applied Sciences, University of Wolverhampton, Wolverhampton, UK
3Peptide Chemistry Department, Chemical Industries Division, National Research Centre, Cairo, Egypt
4Chemistry Department, Faculty of science, Ain Shams University, Cairo, Egypt
5Peptide Chemistry Department, Chemical Industries Research Division, National Research Centre, Cairo, Egypt
6Research Institute in Healthcare Science, School of Applied Sciences, University of Wolverhampton, Wolverhampton, UK.
Abstract
In the interest of public health, cell-penetrating peptides (CPPs) have proven to be important way for the highly efficient intracellular delivery of bioactive cargos like peptides, proteins, and oligonucliotides. It is feasible to combine one or multiple CPPs with bioactive cargos either by direct chemical conjugation or, more rarely, as non-covalent complexes. The term bioportides was introduced to describe monomeric CPPs that are intrinsically bioactive. One of the consistent problems that may compromise the in vivo applications of CPPs and bioportides, is the stimulation of mast cell (MC) secretion. Therefore, in this study two decapeptides, S6 and S6P, has been designed from the c-kit protein, a mast cells receptor, and synthesized using the technology of microwave assisted solid phase peptide synthesis. In an attempt to find bioportides from the c-Kit protein, and studying their structure- activity relationship, the present work was carried out, investigating the effect of the presence of the phosphotyrosine moiety as an active site in the synthesized peptides.
Keywords
Microwave assisted solid phase peptide synthesis; Cell-penetrating peptide; Bioportides; C-Kit receptor
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