Phylogenetic Relationships among Staphylococcus epidermidis based on16S rRNA Gene Sequence | ||
| Egyptian Journal of Medical Microbiology | ||
| Volume 28, Issue 4, October 2019, Pages 157-163 PDF (432.84 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2019.283497 | ||
| Authors | ||
| Shymaa Enany* 1; Samira Zakeer1; Alaa El Din M.S. Hosny2 | ||
| 1Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Egypt | ||
| 2Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Egypt | ||
| Abstract | ||
| Background: Diversity of clones is common in Staphylococci, but the focus on this diversity is less in coagulase negative group. Objective: We aimed to detect the biodiversity of Staphylococcus epidermidis isolated from skin lesions. Methodology: S. epidermidis were identified using Gram stain, catalase and coagulase tests, cultured on mannitol salt agar and tested for antibiotic susceptibility. Biodiversity was assessed using PCR of 16s rRNA genes. Phylogenetic analysis of isolate’s sequences and sequences of S. epidermidis retrieved from Gene bank was done. The sequences were aligned to show their degree of similarity. Results: About 86% and 57% were resistant to penicillin and cefoxitin, fusidic acid, and erythromycin; respectively. Phylogenetic analysis revealed all S. epidermidis involved were belonged to the same cluster with S. epidermidis strain AFATF that was isolated previously from Egypt Conclusion: We confirmed the usefulness of 16s rRNA gene sequence in phylogenetic studies and the biodiversity of our isolates. | ||
| Keywords | ||
| Staphylococcus epidermidis; 16sr RNA; phylogenetic analysis | ||
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