The Study of the Industrial Aptitude of Aspergillus fumigatus Strain for Xylanase Production | ||
| Egyptian Journal of Chemistry | ||
| Volume 66, Issue 10, October 2023, Pages 163-177 PDF (2.23 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2023.174799.7195 | ||
| Authors | ||
| Maroua GARES* 1; HILIGSMANN Serge2; Mahmoud KASSEM AL SAYED3; Wiem ALLOUN1; Noreddine KACEM CHAOUCHE1 | ||
| 1Laboratory of Mycology, Biotechnology and Microbial Activity (LaMyBAM). Department of Applied Biology, FSNV, University of Brothers Mentouri, 25017 Constantine, Algeria. | ||
| 2Biomass Valorisation Platform, Celabor scrl, Avenue du Parc 38, 4650 Herve, Belgium- Belgium | ||
| 3Affiliation 2 : 3BIO-BioTech, Université Libre de Bruxelles ULB, Av. F. Roosevelt 50, CP 165/61, Brussels- Belgium | ||
| Abstract | ||
| The major microorganisms used in the industry are fungi. The production of fungal metabolites on a large scale needs the use of an industrial-scale efficient strain. Indeed, the microorganisms used in an industrial process must be capable of doing more than simply producing the desired product in high yield. In fact, one of the main bottlenecks in the fermentation procedure is the scaling-up process. Indeed, reproducing the best conditions for microorganism growth and metabolism achieved in small cultures when transferring them to a much larger fermentation scale is nearly impossible. Aspergillus fumigatus (ON226990) performance was firstly evaluated in large-scale cultures; batch fermentation using simple sugar (xylose) as substrate in various volumes; 250 mL flasks, 2 L and 20 L bioreactors. The effect of agitation speed on the morphological aspects of this strain was also investigated. Subsequent fermentations were carried out with optimal agitation, using xylan from corncob as substrate in 250 mL flasks and a 2 L bioreactor. Finally, the strain's ability to use low-cost substrate such as Alfa biomass for xylanase production was evaluated as a biotechnological application. | ||
| Keywords | ||
| Bioreactor; Submerged fermentation; Scale-up fermentation; Aspergillus fumigatus; Mycelium morphology | ||
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