Estimation of Tissue Homogenate Cytokines and MicroRNAs might Help to Determine Wound Vitality and Dating | ||
| Zagazig Journal of Forensic Medicine and Toxicology | ||
| Article 9, Volume 21, Issue 1, January 2023, Pages 157-171 PDF (776.41 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/zjfm.2023.182949.1136 | ||
| Authors | ||
| Noha Elnajjar* 1; Maha M. Mokhtar2; Omnia Habashy3; Hebatallah E. Attallah3; Shaymaa M. Abdelrahman3; Sally Elsharkawey2 | ||
| 1Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Benha University | ||
| 2Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Benha University | ||
| 3Medical Biochemistry and Molecular Biology, Faculty of Medicine, Benha University | ||
| Abstract | ||
| Objectives: To assess the applicability of estimated levels of tumor necrosis factor-α (TNF-α) and interleukin (IL)-6, and gene expression levels of microRNAs (Mir) 92a and 214a in tissue homogenate (TH) of skin biopsies harvested from wound for discrimination between antemortem and post-mortem wounds and to suggest the post-injury interval (PII). Experimental Protocol: A 2-cm skin incision was made under anesthesia and full thickness punches were obtained from wound edge immediately (C-group) and at 30-min, 2-h, 6-h and 24-h after wounding in living animals (L-group) or animals were decapitated immediately after wounding and biopsies were obtained at the same periods after decapitation (D-group). Tissues were homogenized to be used for ELISA estimation of TNF-α and IL-6 levels and qRT-PCR expression levels of Mir-92a and 214a. Results: TNF-α, IL-6 and Mir-92a levels were significantly higher in L-group than other groups. Estimated TNF-α and IL-6 levels showed biphasic increases at 30-min and 2h, respectively and at 24h for both, while the peak levels of mir-214a and mir-92a were at 2h and 6h, respectively. MicroRNAs levels showed non-significant differences between all D-group specimens. Regression analysis defined high IL-6 levels as the significant variate to identify PII as either 2h or 24h and high levels of Mir-214a could suggest PII of 2h, while high levels of Mir-92a and TNF-α as the significant variate to suggest PII of 30-min and 6h, respectively. Multivariate analysis defined high IL-6 as the persistently significant predictor for victim's vitality at wounding, while ROC curve analysis defined high Mir-214a levels as the sensitive identifier for victim's viability during wounding. Conclusion: Estimation of expression levels of Mir-92a and 214a in TH might define the probable PII and differentiate antemortem from postmortem wounds, respectively. Combined markers might increase the accuracy of wound-dating. | ||
| Keywords | ||
| Wound dating; Wound vitality; MicroRNAs; Inflammatory cytokines | ||
|
Statistics Article View: 268 PDF Download: 401 |
||
