Sadek, A., Rizkallah, N., Elhosseiny, H., Mahmoud, H., Hanna, G., Sonbol, M. (2022). SHORT AND LONG TERM EFFECTS OF THE TUMOUR NECROSIS FACTOR INHIBITOR ON THE REGENERATION PROCESS OF SCIATIC NERVE TRANSECTION IN ALBINO RAT. test, 73(4), 719-734. doi: 10.21608/asmj.2022.285350
Ashraf M. Sadek; Nawal F. Rizkallah; Hossam M. Elhosseiny; Hoda M. Mahmoud; George F.B. Hanna; Mohamed Moustafa Sonbol. "SHORT AND LONG TERM EFFECTS OF THE TUMOUR NECROSIS FACTOR INHIBITOR ON THE REGENERATION PROCESS OF SCIATIC NERVE TRANSECTION IN ALBINO RAT". test, 73, 4, 2022, 719-734. doi: 10.21608/asmj.2022.285350
Sadek, A., Rizkallah, N., Elhosseiny, H., Mahmoud, H., Hanna, G., Sonbol, M. (2022). 'SHORT AND LONG TERM EFFECTS OF THE TUMOUR NECROSIS FACTOR INHIBITOR ON THE REGENERATION PROCESS OF SCIATIC NERVE TRANSECTION IN ALBINO RAT', test, 73(4), pp. 719-734. doi: 10.21608/asmj.2022.285350
Sadek, A., Rizkallah, N., Elhosseiny, H., Mahmoud, H., Hanna, G., Sonbol, M. SHORT AND LONG TERM EFFECTS OF THE TUMOUR NECROSIS FACTOR INHIBITOR ON THE REGENERATION PROCESS OF SCIATIC NERVE TRANSECTION IN ALBINO RAT. test, 2022; 73(4): 719-734. doi: 10.21608/asmj.2022.285350

SHORT AND LONG TERM EFFECTS OF THE TUMOUR NECROSIS FACTOR INHIBITOR ON THE REGENERATION PROCESS OF SCIATIC NERVE TRANSECTION IN ALBINO RAT

Ain Shams Medical Journal
Article 2, Volume 73, Issue 4, December 2022, Pages 719-734    PDF (1021.84 K)
Document Type: Original Article
DOI: 10.21608/asmj.2022.285350
Authors
Ashraf M. Sadek1; Nawal F. Rizkallah1; Hossam M. Elhosseiny2; Hoda M. Mahmoud1; George F.B. Hanna1; Mohamed Moustafa Sonbol1
1Human anatomy & Embryology Department, Faculty of Medicine, Ain-Shams University, Egypt.
2Neurosurgery Department, Faculty of Medicine, Ain-Shams University, Egypt.
Abstract
Background: In peripheral nerve damage˒ pro-inflammatory cytokines, such as tumour necrosis factor-alpha (TNF- α) are rapidly recruited mediating many of the deleterious events associated with Wallerian degeneration.
Aim of the work: Identify the short and long-term effects of TNF- α inhibitor on the nerve regeneration after experimental sciatic nerve transection. Material and methods: 36 adult male albino rats were divided into 3 groups, 12 rats each. Group A (control) was subdivided into 2 subgroups (A1 & A2). A1 left without any intervention and A2 (sham), half of each subgroup were sacrificed after 1 and 8 weeks. Group B (untreated) was subdivided into 2 subgroups (B1 & B2), sciatic nerve transection was done, B1 was sacrificed after 1 week and B2 after 8 weeks. Group C (treated) was subdivided into two subgroups (C1 & C2), sciatic nerve transection and immediate intraperitoneal injection with anti-TNF- α (Enbrel 25 mg) vial 6 mg/kg single dose was done. C1 was sacrificed after 1 week and C2 after 8 weeks. Nerve endings for all groups were processed for light, transmission electron microscope, immunohistochemical (ED1 cell and S100 antigen), morphometric and statistical studies. Results: 1 week after injury macrophage cells (MC) invasion and excessive demyelination occurred, anti- TNF- α reduced the MC invasion. 8 weeks after the injury regenerating myelinated axons were observed. Number of the regenerating axons showed statistically significant increase in the treated group compared to the injured group.
Conclusion: Immediate administration of TNF- α antagonist after induced sciatic nerve injury enhanced nerve regeneration.
Keywords
Tumour necrosis factor inhibitor; Sciatic nerve transection; Albino rats
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