Risk Prediction Genetic Study of Non-HLA Gene NOD2 Three Polymorphisms in Acute Graft versus Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation | ||
| Egyptian Journal of Medical Microbiology | ||
| Volume 27, Issue 3, July 2018, Pages 135-142 PDF (386.2 K) | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2018.285602 | ||
| Authors | ||
| Hanan F. Abd El-Aziz Ibrahim* 1; Azaa M. Shawky Ghanem1; Maha I. Shehata1; Gamal EL Din M. Fathy2 | ||
| 1Microbiology and Immunology Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo-Egypt | ||
| 2Hematology and Bone Marrow Transplantation Department, Naser Institute, Cairo-Egypt | ||
| Abstract | ||
| Background: Graft-versus-host disease (GVHD) has been associated with non- human leukocyte antigen (HLA) gene polymorphisms after allogenic Hematopoietic stem cell transplantation (aHSCT). Objective: This study aims to investigate the role of three polymorphisms in Nucleotide-binding Oligomerisation Domain (NOD2) gene on the occurrence / severity of acute GVHD among Egyptian population. Methodology: A total of 64 patients and their corresponding donors who underwent aHSCT from HLA-identical matched siblings were genotyped by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Results were confirmed by automated laser sequencer. Results: There was highly statistical significant association between NOD2 recipient l007fs allele and higher incidence of aGVHD p= 0.006. There was no significant association between NOD2 donor l007fs allele, G908R allele in donors and recipients, R702W in donors and recipients with incidence and severity of aGVHD. Conclusion: This research an original pilot study to demonstrate that the NOD2 SNP13 (Leu1007fs) is a possible risk factor for aGVHD among Egyptian population. | ||
| Keywords | ||
| NOD2 polymorphism; GVHD; aHSCT; PCR-RFLP | ||
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