Phytochemicals, Phenolic Compounds and Antioxidant Activity of Garden cress (Lepidium sativum L.) seeds | ||
| Al-Azhar Journal of Agricultural Research | ||
| Articles in Press, Accepted Manuscript, Available Online from 19 February 2023 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ajar.2023.184022.1111 | ||
| Authors | ||
| Mohamed Essam Mohamed Ahmed* 1; MOHAMED MABROUK ELDANASOURY2; Haitham A El-khamissi3; Hanan Anwer Ali Taie4 | ||
| 1Agricultural department, Faculty of agriculture, Alazhar university | ||
| 2Dept. of Agric. Biochemistry, Faculty of Agric. Cairo, Al-Azhar University. | ||
| 3Cairo | ||
| 4Plant Biochemistry Department, Agricultural and Biology Research Institute,National Research Centre, 33 El-Bohouth St. (Former El-Tahrir St.), Dokki 12622, Giza, Egypt | ||
| Abstract | ||
| Garden cress (Lepidium sativum L.) is a rapidly growing annual herb that grows in Ethiopia, Egypt and West Asian countries, although it is now grown all over the world. It is grown for its seeds, as it is the most widely used part. The chemical composition of garden cress (GC) seed powder was examined. The results revealed that GC contains 10.39% moisture, 22.42% crude protein, 25.57% crude fat and 22.19% carbohydrates. Moreover, the recorded ash and fiber content were 9.60% and 9.83%, respectively. Also, phytochemical analysis of GC extracts showed that plant extracts contain tannins, saponins, alkaloids, terpenoids, flavonoids, phenols, phytosterols and sterols. The highest amount of total phenolic was found in the ethanol extract, which gave 25.13 µg/ml of gallic. While, the highest amount of total flavonoids was found in the methanol extract, which gave 41.30 µg/ml of rutin. The HPLC analysis was carried out on the ethanolic and methanolic extracts of garden cress. Seven phenolic acid compounds have been detected in ethanolic extract including Catechol, Syringenic, Cinnamic, Caffeic, Pyrogallol, Gallic and Ellagic, while nine phenolic compounds have been detected in methanolic extract, including Catechol, Syringenic, Cinnamic, Caffeic, Gallic, Ferulic, Salicylic, Ellagic, and Benzoic. Furthermore, the methanol extract of garden cress showed the highest DPPH scavenging activity compared to ethanol extract. It can be concluded that garden cress seeds and their extracts can be used in different fields such as nutritional supplements and medical fields because it is rich in antioxidants. | ||
| Keywords | ||
| Lepidium sativum; phytochemical; total phenolics; total flavonoids; antioxidant activity | ||
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