Screening for the Production of MetNase and Its Optimum Conditions from Streptomycete Isolate | ||
| Egyptian Academic Journal of Biological Sciences, G. Microbiology | ||
| Volume 15, Issue 1, June 2023, Pages 77-86 PDF (640.42 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsg.2023.289460 | ||
| Authors | ||
| Mohamed O. Abdelmonem1; Mervat G. Hassan1; Mohamed E. Elawady2; Manal M. Mohamed2; Reem I. Hilal1 | ||
| 1Department of Botany and Microbiology, Faculty of Science, Benha University, Benha 33516, Egypt | ||
| 2Department of Genetic Engineering and Biotechnology National Research Center. | ||
| Abstract | ||
| MetNase might be a valuable source for different medical applications. MetNase has the potential to be used against a wide range of tumour cell types as an anticancer agent. The purpose of this research is to screen and develop optimal conditions of streptomycetes isolates for MetNase production. For this study, 34 randomly selected streptomyces isolates from soil and marine water were used. The isolates that can produce MetNase were qualitatively selected using a quick plate assay method. When the pH was set to 7.0, the incubation temperature was set to 30 °C, and the carbon and nitrogen sources were glucose and peptone, respectively, the highest levels of MetNase synthesis were attained. | ||
| Keywords | ||
| Streptomycetes; MetNase; isolation; screening; optimization | ||
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