Abdel ghaffar Rezk, D., sarhan, M., abdel moniem, H., abbas, A., salama, M. (2019). Possible role of angiogenesis suppression in rats model of non alcoholic fatty liver disease. test, 39(2), 173-190. doi: 10.21608/besps.2019.4410.1004
Dalia Mohammed Abdel ghaffar Rezk; mohammed el mahdy sarhan; hanaa ahmed abdel moniem; amr medhat abbas; mohammad eldosoky salama. "Possible role of angiogenesis suppression in rats model of non alcoholic fatty liver disease". test, 39, 2, 2019, 173-190. doi: 10.21608/besps.2019.4410.1004
Abdel ghaffar Rezk, D., sarhan, M., abdel moniem, H., abbas, A., salama, M. (2019). 'Possible role of angiogenesis suppression in rats model of non alcoholic fatty liver disease', test, 39(2), pp. 173-190. doi: 10.21608/besps.2019.4410.1004
Abdel ghaffar Rezk, D., sarhan, M., abdel moniem, H., abbas, A., salama, M. Possible role of angiogenesis suppression in rats model of non alcoholic fatty liver disease. test, 2019; 39(2): 173-190. doi: 10.21608/besps.2019.4410.1004

Possible role of angiogenesis suppression in rats model of non alcoholic fatty liver disease

Bulletin of Egyptian Society for Physiological Sciences
Article 4, Volume 39, Issue 2, December 2019, Pages 173-190    PDF (1.11 M)
Document Type: Original Article
DOI: 10.21608/besps.2019.4410.1004
Authors
Dalia Mohammed Abdel ghaffar Rezk* 1; mohammed el mahdy sarhan2; hanaa ahmed abdel moniem3; amr medhat abbas3; mohammad eldosoky salama4
1physiology department, faculty of medicine, mansoura university, talkha city, dakahlyia gov.
2physiology depatment, Faculty of Medicine, Mansoura University, Masoura, Egypt.
3physiology department, faculty of medicine, mansoura university, mansoura, eygpt
4physiology department, faculty of medicine, mansoura university, mansoura, eygpt.
Abstract
Objective:to evaluate development of angiogenesis in rats of NAFLD and to determine the protective effects of antiangiogenic therapy (sorafenib) in preventing the progression of NAFLD.
Methods: 45 Albino rats (200-300 g) were divided into 3 groups (15 rats for each): Group I: Control group fed on an ordinary diet. Group II: rats received high fat, high fructose diet (HFD,HFr) with DEN ( twice weekly for 8 weeks, ip). Group III: rats received HFD, HFr + DEN + sorafenib orally for 8 weeks. Biochemical, histopathologial, and immunohistopathological examination were studied.
Results: high fat, high fructose diet with DEN resulted in a significant elevation in the serum cholesterol, TG, LDL, and AST, and ALT, significantly lower levels of HDL and Albumin together with a significant decrease in hepatic GSH, Histopathological examination revealed that liver of untreated rats showed severe fatty infilteration (grade3). Immunohistochemical examination of liver of untreated NAFLD rats showed strong staining reactions against VEGF, α- SMA, CD31, and Caspase3 antibodies. Oral administration of sorafenib alleviated all these parameters.
Keywords
Angiogenesis; Non alcoholic fatty liver disease (NAFLD); Sorafenib; vascular endothelial growth factor (VEGF)
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