Evaluating the therapeutic effect of Diallyl disulfide compared to that of Alderonate on Glucocorticoids induced osteoporosis in rats: Biochemical and histomorphometric analysis | ||
| Bulletin of Egyptian Society for Physiological Sciences | ||
| Article 1, Volume 39, Issue 2, December 2019, Pages 129-142 PDF (834.25 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/besps.2019.6704.1011 | ||
| Authors | ||
| Noha Badae* 1; Rasha Abdelmawla Ghazala2; Eiman Ibrahim Zaki3; Suzan Awad Abdel-Ghani4 | ||
| 1Physiology,Faculty of medicine, Alexandria University | ||
| 2Medical Biochemistry department, Faculty of Medicine, Alexandria University | ||
| 3Histology and Cell Biology department, Faculty of Medicine, Alexandria University | ||
| 4Clinical pharmacology department, Faculty of Medicine, Alexandria University | ||
| Abstract | ||
| Introduction: The prevalence of Glucocorticoids (GC) use made Glucocorticoid induced osteoporosis (GIO) an important form of secondary osteoporosis. Bisphosphonates (Alderonate) are considered as pharmacological agents in prevention and treatment of GIO. Garlic containing Diallyl disulfide (DAS) has received special attention for its beneficial effects as an antioxidant. The present study attempted to evaluate the Alendronate, DAS, and the combination of Alderonate and DAS effect on bone gene expression of RANKL and OPG, serum biochemical parameters [Ca, P, alkaline phosphatase (ALP)] and histological assessment of tibia in animal model of GIO. Material and Method: Fifty pathogen albino male rats were allocated in five groups:Control group(C), Methyl prednisolone group (M), methyl prednisolone Aldenronate group (A) Methyl prednisolone Diallyl disulfide (D), Diallyl disulfide Alendronate Methyl prednisolone (AD) group.Gene expression studies, biochemical parameters, histological and morphometric studies were assessed for all animals. Results: Among the study groups, Methyl prednisolone exposure provoked decrease in OPG (0.26±0.16) increase in RANKL (3.57±.39) gene expression, decrease in serum ALP(85.38±6.3),Ca(6.41±0.89), P(1.9 ±0.35) levels and decrease in trabecular thickness (57.01±23.22).Alderonate and Dially disulfide concomitant administration was shown to increase OPG(2.84 ±0.53)and decrease in RANKL (0.63 ± 0.27) gene expression, increase serum ALP(187.75±24.93),Ca(10.330 ±.69)and P(3.16 ±0.43) levels,increase trabecular thickness(154.7±31.7)(p < 0.001). Conclusion: Diallyl disulfide can add advantage to Alderonate in treatment of glucocorticoid induced osteoprosis. | ||
| Keywords | ||
| Osteoporosis; Alendronate; Diallyl disulfide, OPG, RANKL | ||
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