Fahim, J., Abdelhafez, O., AboulMagd, A., Abdelmohsen, U., desoukey, S., Kamel, M. (2023). In vitro cytotoxic potential of Nephthea sp. and its silver nanoparticles against hepatic and colon cancer cells assisted with molecular docking studies. test, 6(2), 86-89. doi: 10.21608/jabps.2023.184564.1178
John Refaat Fahim; Omnia Hisham Abdelhafez; Asma M. AboulMagd; Usama Ramadan Abdelmohsen; samar yehia desoukey; Mohamed Salah Kamel. "In vitro cytotoxic potential of Nephthea sp. and its silver nanoparticles against hepatic and colon cancer cells assisted with molecular docking studies". test, 6, 2, 2023, 86-89. doi: 10.21608/jabps.2023.184564.1178
Fahim, J., Abdelhafez, O., AboulMagd, A., Abdelmohsen, U., desoukey, S., Kamel, M. (2023). 'In vitro cytotoxic potential of Nephthea sp. and its silver nanoparticles against hepatic and colon cancer cells assisted with molecular docking studies', test, 6(2), pp. 86-89. doi: 10.21608/jabps.2023.184564.1178
Fahim, J., Abdelhafez, O., AboulMagd, A., Abdelmohsen, U., desoukey, S., Kamel, M. In vitro cytotoxic potential of Nephthea sp. and its silver nanoparticles against hepatic and colon cancer cells assisted with molecular docking studies. test, 2023; 6(2): 86-89. doi: 10.21608/jabps.2023.184564.1178

In vitro cytotoxic potential of Nephthea sp. and its silver nanoparticles against hepatic and colon cancer cells assisted with molecular docking studies

Journal of Advanced Biomedical and Pharmaceutical Sciences
Article 4, Volume 6, Issue 2, April 2023, Pages 86-89    PDF (373.63 K)
Document Type: Original Article
DOI: 10.21608/jabps.2023.184564.1178
Authors
John Refaat Fahim* 1; Omnia Hisham Abdelhafez2; Asma M. AboulMagd3; Usama Ramadan Abdelmohsen4; samar yehia desoukey5; Mohamed Salah Kamel6
1Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt
2Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, New Minia 61111, Egypt.
3Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Nahda University, Beni Suef 62513, Egypt.
4Department of Pharmacognosy, faculty of Pharmacy, Minia University, 61519 Minia, Egypt
5Pharmacognosy department Faculty of Pharmacy Minia University
6Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt.
Abstract
The current study aimed to evaluate the cytotoxic potential of the total extract of the soft coral Nephthea sp. and its derived fractions (petroleum ether, ethyl acetate, n-butanol and acetone) in addition to their green synthesized silver nanoparticles (AgNPs) against hepatocellular carcinoma (HepG2) and colon adenocarcinoma (HT29) cell lines. Of these, the petroleum ether fraction exhibited potent inhibitory activity against HepG2 cells, with an IC50 value of 1.10 ± 0.03 μg/ml, while the n-butanol fraction revealed significant cytotoxic potential against HT29 cells (IC50= 3.69 ± 0.16 μg/ml). On the other hand, the prepared AgNPs of both the total extract and its ethyl acetate fraction demonstrated the maximum inhibitory effects towards HepG2 and HT29 tumor cells, respectively (IC50= 0.35 ± 0.01 and 8.97 ± 0.48 μg/ml, respectively). Moreover, an in-silico molecular docking analysis of a group of 14 identified metabolites from the petroleum ether fraction showed the considerable binding affinity of compounds 1 (nephthoside monoacetate), 5 (chabrolobenzoquinone E), 10 (chabrolohydroxybenzoquinone E), and 12 (2-N-nonanoyl-4,5-dihydrosphingosine) to the common tyrosine kinase, epidermal growth factor receptor (EGFR), which might suggest their contribution to the observed cytotoxic potential of this fraction of Nephthea sp. These results could therefore pave the way towards further search for varied antitumor candidate metabolites from Nephthea sp.
Keywords
Cytotoxicity; EGFR; Molecular docking; Secondary metabolites; Soft corals
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