Ahmed, A., Abd El-Aziz, G., Fouad, H., Riad, H. (2023). Molecular signaling pathways in an experimental model of Cardiorenal Syndrome. test, 1(1), 7-18. doi: 10.21608/ejctr.2023.196319.1005
Arwa Mokhtar Ahmed; Ghada Mahmoud Abd El-Aziz; Hanan Hassan Fouad; Hoda Ramadan Riad. "Molecular signaling pathways in an experimental model of Cardiorenal Syndrome". test, 1, 1, 2023, 7-18. doi: 10.21608/ejctr.2023.196319.1005
Ahmed, A., Abd El-Aziz, G., Fouad, H., Riad, H. (2023). 'Molecular signaling pathways in an experimental model of Cardiorenal Syndrome', test, 1(1), pp. 7-18. doi: 10.21608/ejctr.2023.196319.1005
Ahmed, A., Abd El-Aziz, G., Fouad, H., Riad, H. Molecular signaling pathways in an experimental model of Cardiorenal Syndrome. test, 2023; 1(1): 7-18. doi: 10.21608/ejctr.2023.196319.1005

Molecular signaling pathways in an experimental model of Cardiorenal Syndrome

Egyptian Journal of Cell and Tissue Research
Volume 1, Issue 1, January 2023, Pages 7-18    PDF (668.04 K)
Document Type: Original articles
DOI: 10.21608/ejctr.2023.196319.1005
Authors
Arwa Mokhtar Ahmed* 1; Ghada Mahmoud Abd El-Aziz1; Hanan Hassan Fouad2; Hoda Ramadan Riad1
1Medical Biochemistry and Molecular Biology department, Faculty of Medicine, Beni-Suef University, Egypt
2Medical Biochemistry and Molecular Biology department kasralainy Faculty of Medicine Cairo University
Abstract
The goal of this study is to investigate the role of the fibroblast growth factor 23 (FGF23) / Klotho (Klotho) molecular signaling axis in an experimental model of cardiorenal syndrome type 3 resulting from right renal artery ligation leading to renal reperfusion ischemia. The study used 12 male Wistar rats (6-8 months old, weighing 230-300g) split into 2 groups of 6 rats each (Group I, controls) and Group II, diseases, where Cardiac Hypertrophy was created as a result of renal ischemia by ligating the right renal artery. Gene expression of FGF23 and Klotho in cardiac and renal tissue was measured using quantitative real-time PCR, and blood urea and creatinine levels were measured clinically.All afflicted rats had increased amounts of creatinine in their blood, and histological analysis revealed renal ischemia and heart hypertrophy. According to real time PCR, FGF23 expression has dramatically increased in cardiac and renal tissues whereas alpha klotho expression has decreased.
Keywords
cardiorenal; klotho; FGF23
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