Evaluation of Nandrolone Decanoate Effects on Sinoatrial Node in Experimental Rat Model (Histological and Immunohistochemical study) | ||
| Journal of Medical Histology | ||
| Article 1, Volume 5, Issue 2, December 2021, Pages 93-104 PDF (2.62 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jmh.2022.170705.1105 | ||
| Authors | ||
| Marwa El Sawy* ; Reham Tash; Ahmed Desouky | ||
| Department of Anatomy, Faculty of Medicine, Ain Shams University, Egypt. | ||
| Abstract | ||
| Background: Nandrolone decanoate (NA) is one of the anabolic steroids used medically and abused by athletes but has several harmful effects. Aim: Evaluate the effect of nandrolone decanoate on sinoatrial node structure of albino rat and the effect of its withdrawal. Materials and Methods: Thirty adult albino rats were divided into three groups each containing ten rats. Group I (control): ten rats were divided into the negative control was left untreated and the positive control was injected by castor oil, twice weekly/8 weeks. Group II (NA group): ten rats were injected by NA 5 mg/kg twice/week/8 weeks. Group III (recovery group): ten rats were injected by NA 5 mg/kg twice/week /8 weeks, then were left 12 weeks to evaluate recovery. At the end, the sinoatrial node were dissected out, and subjected to histological and immunohistochemical examination. Results: Examination of right atrial sections of NA injected group showed wide separation of sinoatrial node structure and disordered cell arrangement. P cell degeneration with increased cytoplasmic vacuoles was noted. Some cardiomyocytes exhibited degenerative changes including wavy sarcoplasm and fragmented pyknotic nuclei. After discontinuation of NA, the sinoatraial node did not regain its normal architecture. Mean area percentage % of collagen fiber deposition, SYN and iNOS immunoexpressing in NA injected group was significantly increased relative to control group which did not recovered after drug withdrawal. Conclusion: Use of NA caused serious changes in sinoatrial node tissue which will threaten life of their users especially the athletes. Thus, NA must be administered only under medical supervision. | ||
| Keywords | ||
| Nandrolone decanoate; rat; recovery; sinoatrial node | ||
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