Therapeutic Efficacy of Melatonin Pretreated Bone Marrow Mesenchymal Stem Cells Versus Mesenchymal Stem Cells in Experimental Diabetic Cardiomyopathy in Rat | ||
| Journal of Medical Histology | ||
| Article 4, Volume 5, Issue 2, December 2021, Pages 134-153 PDF (8.19 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jmh.2022.165071.1104 | ||
| Authors | ||
| Heba Elnegris* 1; Sahar Khalil2; Dahlia Badran3; Rania Galhom4 | ||
| 1Department of Histology and Cell Biology, Faculty of Medicine, Zagazig University, Zagazig, Badr University in Cairo, Cairo, | ||
| 2Suez Canal University, Ismailia, Department of Biochemistry Molecular Biology, Faculty of Medicine, | ||
| 3Suez Canal University, Ismailia, & Badr University in Cairo, Cairo, Department of Human Anatomy and Embryology, | ||
| 4Suez Canal University, Ismailia, Badr University in Cairo, Cairo, &Tissue Culture Unit, Center of Excellence in Molecular and Cellular Medicine (CEMCM), Suez Canal University. Ismailia, Egypt | ||
| Abstract | ||
| Background: Diabetic cardiomyopathy (DCM) is a common condition that is associated with morbidity and mortality. With the medical advancement in cell treatment, stem cell therapy has a potential therapeutic strategy for DCM. Objectives: We aim to evaluate the possible therapeutic effects of Mesenchymal stem cells (MSCs) versus melatonin (MLT) preconditioned-MSCs in induced diabetic cardiomyopathy in rats. Materials and Methods: Forty-five male adult albino rats were divided into four groups. Group I: Control group. Group < br />II: Diabetic group: Rats received single intraperitoneal injection of streptozotocin STZ (50 mg/kg body weight). Group < br />III: DC treated with MSCs. Group IV: DC treated with MLT preconditioned-MSCs. Histological, ultrastructural and immunohistochemical studies were performed on left ventricle myocardium. We also estimated the survival of MLTpreconditioned MSCs in vitro. Bcl-2, Casp-3, interleukin 1 (IL-1), and interleukin 10 (IL-10) expression were measured using real-time PCR in tissue homogenates. Results: Our findings revealed a significant increase in immunohistochemical expression of apoptotic markers in the diabetic group, as well as a disruption of the normal cardiac histological ultrastructure. Melatonin improved MSCs survival in vitro and altered apoptotic markers. In addition, the melatonin-preconditioned MSC group showed a significant increase in B-cell lymphoma 2 (Bcl-2) mRNA expression and a significant decrease in Casp-3. Enhanced immunomodulatory behavior was obvious by a significant attrition of the mRNA expression of IL-1β (inflammatory-agonist) and a significant altitude of IL-10 (inflammatory-antagonist). Conclusion: The melatonin-preconditioning of MSCs enhance their survival and immunomodulatory activities and precisely restored cardiac histology. Thus, it unveils an encouraging interference of DC. | ||
| Keywords | ||
| Albino rats; Melatonin; Mesenchymal stem cells; Myocardial; Type II Diabetes mellitus | ||
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