Immune response of Enterobacter aerogenes ghost vaccine in mice | ||
| Biochemistry Letters | ||
| Volume 19, Issue 1, 2023, Pages 1-11 PDF (701.75 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/blj.2023.294038 | ||
| Authors | ||
| Faten Zahran Mohammed1; Akaber Tarek Keshta1; Mahmoud Ayman Hassan1; Hossam Ghoneim2; Amro A Amara3; Salah Ahmed Sheweita4, 5 | ||
| 1Department of Chemistry (Biochemistry Division, Faculty of Science, Zagazig University, Egypt | ||
| 2Department of Immunology and Allergy, Medical Research Institute, Alexandria University, Egypt | ||
| 3Department of Protein Research, City of Scientific Research and Technological Applications, Alexandria, Egypt | ||
| 4Department of Clinical Biochemistry, Faculty of Medicine, King Khalid University, Abha, KSA. | ||
| 5Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Egypt | ||
| Abstract | ||
| Background: The emergence of multidrug-resistant Enterobacter aerogenes has become a public health concern. E. aerogenes is a common cause of hospital-acquired infections such as pneumonia and urinary tract infections. Aim: This study focused on the use of E. aerogenes ghosts as a potential vaccine against the opportunistic pathogen E. aerogenes. Methods: This study used a cost-effective method to generate the E. aerogenes ghosts using certain chemicals. The safety and effectiveness of the ghosts were evaluated in a mouse model by administering three doses over a 14-day period. The mice were then challenged with a live E. aerogenes strain. Results: The results of the cytokine assays showed that vaccinated mice produced higher amounts of IL-10 and TNF-α than did control saline mice, indicating a good immune response. Additionally, the results of the liver function tests indicated that there was a decrease in the activities of ALT and AST in the vaccinated mice as compared to the non-vaccinated mice and low levels of TP and Alb in non-vaccinated mice, suggesting a reduced liver injury and inflammation in vaccinated mice. Conclusion: Overall, the study suggests that E. aerogenes ghosts may be a promising candidate for use as a vaccine against E. aerogenes infection. | ||
| Keywords | ||
| Bacterial ghost; Enterobacter aerogenes; Vaccine; Immune response; Cytokines | ||
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