Diab, N., shiha, G., fareed, Y., Zahran, F. (2023). Potential of mesenchymal stem cells conditioned media from different sources acts as antitumor in vivo and in vitro.. test, 19(1), 12-26. doi: 10.21608/blj.2023.294039
Nervana S. Diab; Gamal shiha; Yasmine fareed; Faten Zahran. "Potential of mesenchymal stem cells conditioned media from different sources acts as antitumor in vivo and in vitro.". test, 19, 1, 2023, 12-26. doi: 10.21608/blj.2023.294039
Diab, N., shiha, G., fareed, Y., Zahran, F. (2023). 'Potential of mesenchymal stem cells conditioned media from different sources acts as antitumor in vivo and in vitro.', test, 19(1), pp. 12-26. doi: 10.21608/blj.2023.294039
Diab, N., shiha, G., fareed, Y., Zahran, F. Potential of mesenchymal stem cells conditioned media from different sources acts as antitumor in vivo and in vitro.. test, 2023; 19(1): 12-26. doi: 10.21608/blj.2023.294039

Potential of mesenchymal stem cells conditioned media from different sources acts as antitumor in vivo and in vitro.

Biochemistry Letters
Volume 19, Issue 1, 2023, Pages 12-26    PDF (703.46 K)
Document Type: Original Article
DOI: 10.21608/blj.2023.294039
Authors
Nervana S. Diab1; Gamal shiha2, 3; Yasmine fareed4; Faten Zahran4
1Children hospital, Faculty of medicine, Mansoura university, Mansoura, Egypt.
2Egyptian liver research institute and hospital (ELRIAH), Mansoura, Egypt.
3Hepatology and gastroenterology Unit, Internal Medicine Department, Faculty of Medicine,Mansoura university, Mansoura, Egypt.
4Biochemistry Division، Chemistry Department، Faculty of science، Zagazig University، Zagazig, Egypt.
Abstract
Background: Mesenchymal stem cell conditioned media (MSCs-CM) is derived from MSC culture and contains the majority of the potential cytokines secreted by MSCs. Ehrlich ascites carcinoma (EAC), a transplantable neoplasia derived from a malignant epithelium, causes ascites when it is injected into the intraperitoneal cavity. Aim: the present study revealed antitumor potential of MSCs-CM from different sources on experimental animals tumor model (EAC) in vivo and on cancer cell line (MCF-7) in vitro Material and methods: Sixty female mice were divided into 6 groups (n=10) all groups except group 1 received EAC cells (1×10 6). Group 1 negative control group, group 2: positive EAC control group, group 3: MTX treated group. Group 4: adipose MSCS-CM treated group, group 5: liver MSCs-CM treated group and group 6: bone marrow treated group. At the end of the experiment:  liver, kidney tissues and ascites fluid were collected. Results: Treatment with MSCs-CM showed a significant decrease in tumor cell count and reduced in ascites volume moreover a significant decrease in malondialdhyde levels compared to positive EAC group (p<0.001) while a significant increase in levels of total antioxidant capacity had been detected compared to EAC group (p<0.001). Whereas in human breast cell line (MCF-7), marked induction of apoptosis was observed.  Conclusion: MSCs-CM has antitumor activity in vivo and in vitro.
 
Keywords
Cancer; Conditioned media; liver; Adipose tissue; Bone marrow
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