.Silver Nanoparticles Attenuate Inflammation aggravation in Hepatocellular Carcinoma in Rats. | ||
| Arab Journal of Nuclear Sciences and Applications | ||
| Volume 56, Issue 4, July 2023, Pages 73-80 PDF (698.73 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ajnsa.2023.180669.1698 | ||
| Authors | ||
| mahmoud refaat1; Shadia Fathy2; Mohamed Ragaa2; soheir osman1; Amany Raafat3; Asmaa Hassan* 4 | ||
| 1Radiation Biology Department, National Centre for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt | ||
| 2Biochemistry department, faculty of science, Ain Shams University, Cairo, Egypt | ||
| 3Chemistry Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority (AEAE), P.O. Box 29, Nasr City 11731, Cairo, Egypt | ||
| 4Department of Radiation Biology, National Center of Radiation Research and Technology, Atomic Energy Authority, Egypt | ||
| Abstract | ||
| Using silver nanoparticles is a promising tactic in healthcare and cancer therapy, either alone or as an adjuvant in traditional chemotherapy. Also, using gamma radiation in nanoparticle preparation is simple, powerful, and environmentally friendly. This study aimed to assess the impact of silver nanoparticles (AgNPs) prepared by gamma radiation (γ-radiation) on hepatocellular carcinoma (HCC) induced by diethyl-nitrosamine (DEN). MTT Cytotoxicity assay of AgNPs has been detected in vitro on Wi38 normal fibroblast and HepG2 hepatocellular carcinoma cell lines. Twenty-eight adult male albino rats were divided into four groups. After HCC development, rats received (10µg/g b. wt.) of AgNPs, three times a week for four weeks liver enzymes were investigated. TNF-α, TGF-β, and IL-1β inflammatory markers were detected. Also, the transcriptional factor (NF-κB) was calculated. The obtained results confirmed the selective influence of AgNPs on the HCC cell line in a time and dose-dependent manner. Moreover, the role of AgNPs is documented in diminishing the inflammation accompanied by HCC development through decreased inflammatory markers and increased apoptosis. | ||
| Keywords | ||
| Hepatocellular carcinoma; silver nanoparticles; gamma radiation; diethyl-nitrosamine | ||
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