Multidrug Resistance of Gallibacterium anatis Isolated from Layer Chickens | ||
| Suez Canal Veterinary Medical Journal. SCVMJ | ||
| Articles in Press, Accepted Manuscript, Available Online from 16 April 2023 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/scvmj.2023.196104.1122 | ||
| Authors | ||
| Marwa El Sayed Abo Hashem* 1; Mohamed El Sayed Enany2; Ahmed Mohamed Ammar3; Ahmeed Hamouda4; basma fathy moawed5 | ||
| 1Bacteriology, Immunology,Mycology. Faculty of Veterinary Medicine. Suez Canal University | ||
| 2Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Suez Canal University | ||
| 3Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University | ||
| 4Microbiology Department, Animal Health Research Institute, Zagazig Branch, Zagazig, Egypt. | ||
| 5depatment of microbiology, animal health research institute , zagazig branch | ||
| Abstract | ||
| Background: Gallibacterium anatis (G. anatis) is a Gram-negative bacterium of the Pasteurellaceae family that resides normally in the respiratory and reproductive tracts in poultry. It is a major cause of oophoritis, salpingitis, and peritonitis, decreases egg production and increased mortalities in layers. The aim of the current study was to detect the prevalence of G. anatis in layer chickens and antimicrobial sensitivity testing of the recovered isolates. Methods: A total of 400 samples (100 from cloacal swabs, 100 from tracheal swabs, 100 from lung, 100 from ovary and oviduct) were collected randomly from private commercial layers flocks with high mortality at El-Sharkia Governate, Egypt. Collected samples were subjected for bacteriological examination for isolation of G. anatis bacteria. The recovered isolates were tested for antimicrobial sensitivity testing and detection of the prevalence of multidrug-resistant (MDR) strains. Results: From 400 diseased examined samples, 120 were positive for G. anatis as follows (cloacal swabs 27/100; 27%, tracheal swabs 35/100; 35%, lung 35/100; 35% and ovary and oviduct 23/100; 23%) of layer chickens. The overall prevalence of G. anatis was 30% in the examined samples. The recovered isolates were highly resistant to doxycycline, amoxycillin and gentamycin with 98%, 96% and 95%, respectively. All isolates were MDR. Isolates were sensitive to florfenicol (90%), erythromycin (96%), difloxacin (44%) and sulfamethoxazole- trimethoprim (57%). Conclusion: The current study revealed the serious and wide prevalence of MDR G. anatis in poultry. The retrieved isolates were highly resistant for amoxycillin, doxycycline and gentamycin which make a serious problem in poultry industry and considered a public health alarm. | ||
| Keywords | ||
| G. anatis; poultry; MDR; antimicrobial sensitivity testing | ||
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