In vitro maturation (IVM) of camel oocytes using different maturation media | ||
| Benha Veterinary Medical Journal | ||
| Volume 44, Issue 1, April 2023, Pages 30-33 PDF (863.03 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bvmj.2023.168049.1604 | ||
| Authors | ||
| Shimaa Adel Gwaid* 1; Ahmed Reda2; Mohsen Aggag3; Alaa Abdelghafar4 | ||
| 1Theriogenology, Benha university, Benha, Egypt | ||
| 2Theriogenology department, Faculty of Veterinary Medicine, Benha University | ||
| 3Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Egypt. | ||
| 4Theriogenology department -benha university- faculty of veterinary medicine. | ||
| Abstract | ||
| The aim of the present study is to evaluate the in vitro maturation rate of camel oocytes using different maturation media. Ovaries of 25 she-camel were collected during the breeding season (from December to April). Ovaries were transferred to the lab in a thermal flask containing warm saline solution within 2 hrs. after slaughtering. Oocytes were collected using 2 techniques; aspiration of graafian follicles and slicing of the ovaries. Depending on the morphological quality, the collected oocytes were selected and cultured on different types of maturation media for 30-40 hours at 38.5˚c, 5% CO2, and maximum humidity (95-96%). The obtained results revealed that the slicing technique produced a higher oocyte recovery rate compared to the aspiration technique. In addition, the maturation media composition mainly the FSH affects the in vitro maturation rate of camel oocytes. It could be concluded that the media modification used in oocytes maturation might be one of the factors that improve the rate of oocyte maturation. | ||
| Keywords | ||
| Dromedary camel; maturation media; oocyte maturation; oocyte retrieval | ||
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