CRYPTOSPORIDIOSIS AMONG OUTPATIENTS WITH DIARRHEA IN EL FAYOUM GENERAL HOSPITAL EGYPT: DIAGNOSIS AND RISKS | ||
| Journal of the Egyptian Society of Parasitology | ||
| Volume 53, Issue 1, April 2023, Pages 57-62 PDF (854.44 K) | ||
| DOI: 10.21608/jesp.2023.297344 | ||
| Authors | ||
| FATEN AHMED M. MOHAMED1; MOHAMED MASOUD2 | ||
| 1Department of Medical Parasitology | ||
| 2Department of Public Health, Faculty of Medicine, Fayoum University, Egypt | ||
| Abstract | ||
| Cryptosporidium is a significant zoonotic parasite causing a self-healing gastroenteritis; with C. hominis and C. parvum are the main agents of cryptosporidiosis. This study examined stool samples from diarrheic patient live in rural areas in Fayoum Governorate, to identify causative species and risky factors associated with zoonosis. Of 152 stool samples were collected from December 2020 to February 2021 from outpatient clinics in El Fayoum General Hospital. Screened smeared by MZN stain detected oocysts in 26 samples, and Copro-ELISA assay detected positive in 32 (21.05%). By nested PCR targeting 18s rRNA gene, 11 samples were amplified. Cryptosporidium genotyping by REFLP showed predominance of C. parvum genotype followed by C. hominis. This was supported by highly risky association (P ≤ 0.05, OR =0.186 [0.061-0.562]) found between infected patients and animals contact. | ||
| Keywords | ||
| El-Fayoum; Outpatients; Cryptosporidiosis; Diagnosis; Risk factor | ||
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