ABU JABEL, O., ROGAN, M. (2023). INVESTIGATION ON THE VARIABILITY OF HYDATID CYST FLUID AS AN ANTIGEN FOR THE IMMUNODIAGNOSIS OF CYSTIC HYDATID DISEASE. test, 53(1), 141-150. doi: 10.21608/jesp.2023.297360
OMYMA M. ABU JABEL; MICHAEL T. ROGAN. "INVESTIGATION ON THE VARIABILITY OF HYDATID CYST FLUID AS AN ANTIGEN FOR THE IMMUNODIAGNOSIS OF CYSTIC HYDATID DISEASE". test, 53, 1, 2023, 141-150. doi: 10.21608/jesp.2023.297360
ABU JABEL, O., ROGAN, M. (2023). 'INVESTIGATION ON THE VARIABILITY OF HYDATID CYST FLUID AS AN ANTIGEN FOR THE IMMUNODIAGNOSIS OF CYSTIC HYDATID DISEASE', test, 53(1), pp. 141-150. doi: 10.21608/jesp.2023.297360
ABU JABEL, O., ROGAN, M. INVESTIGATION ON THE VARIABILITY OF HYDATID CYST FLUID AS AN ANTIGEN FOR THE IMMUNODIAGNOSIS OF CYSTIC HYDATID DISEASE. test, 2023; 53(1): 141-150. doi: 10.21608/jesp.2023.297360

INVESTIGATION ON THE VARIABILITY OF HYDATID CYST FLUID AS AN ANTIGEN FOR THE IMMUNODIAGNOSIS OF CYSTIC HYDATID DISEASE

Journal of the Egyptian Society of Parasitology
Volume 53, Issue 1, April 2023, Pages 141-150    PDF (978.09 K)
DOI: 10.21608/jesp.2023.297360
Authors
OMYMA M. ABU JABEL1; MICHAEL T. ROGAN2
1Department of Biology, Faculty of Science, Jazan University, Saudi Arabia
2School of Environment and Life Sciences, University of Salford, Salford, UK
Abstract
Cystic echinococcosis (CE) affects humans and animals worldwide. Zoonotic diagnoses depend
on imaging primarily ultrasound and immunodiagnostic techniques, which are costeffective
tools for epidemiological studies.
The current study investigated the utilization of hydrated cyst fluids (HCF) as diagnostic antigens,
which were collected from various intermediate hosts. Sixteen crude HCF samples from
humans, sheep, gerbils, horses, and cows were evaluated for their reactivity with human sera
using an enzyme-linked immunosorbent assay (ELISA). The Bradford protein assay was used
to assess the total protein concentration of each sample of cyst fluid. Sodium dodecyl sulphate
polyacrylamide gel electrophoresis (SDS-PAGE) was used to separate the protein contained in
the HCF of the samples. Immunoblotting was used to carry out antigen recognition. The HCF
of the several intermediate hosts varied in their reactivity, according to ELISA results and this
variation was unrelated to the protein content in the HCF samples. HCF samples' SDS-PAGE
profiles revealed variance in several proteins and existence of 12 distinct protein bands ranging
in size from 8 to 148 KDa. There were 98 & 64 KDa proteins found in all HCF samples.
Bovine serum albumin was the 98KDa protein. In comparison, 19% of the fluid samples included
an 8KDa protein indicative by AgB, while 50% of HCF samples contained a 50KDa
protein. DS-PAGE profiles' proteins didn't show Ag5. The 64KDa protein was detected with
potential for application in the diagnosis of human CE, according to immunoblot results.
Keywords
Hydatid cyst fluid; ELISA; SDS-PAGE; Immunoblot; Host
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