Ali, A., Ahmed, S., Abdu-Allah, G., El-Sagheer, S. (2023). Effect of Some Herbicides on the Total Seed Proteins Patterns of Maize and Sorghum Using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS–PAGE). test, 54(2), 190-201. doi: 10.21608/ajas.2023.187545.1221
Ahmed H. Ali; Sayed A. Ahmed; Gamal A.M. Abdu-Allah; Soliman M. El-Sagheer. "Effect of Some Herbicides on the Total Seed Proteins Patterns of Maize and Sorghum Using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS–PAGE)". test, 54, 2, 2023, 190-201. doi: 10.21608/ajas.2023.187545.1221
Ali, A., Ahmed, S., Abdu-Allah, G., El-Sagheer, S. (2023). 'Effect of Some Herbicides on the Total Seed Proteins Patterns of Maize and Sorghum Using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS–PAGE)', test, 54(2), pp. 190-201. doi: 10.21608/ajas.2023.187545.1221
Ali, A., Ahmed, S., Abdu-Allah, G., El-Sagheer, S. Effect of Some Herbicides on the Total Seed Proteins Patterns of Maize and Sorghum Using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS–PAGE). test, 2023; 54(2): 190-201. doi: 10.21608/ajas.2023.187545.1221

Effect of Some Herbicides on the Total Seed Proteins Patterns of Maize and Sorghum Using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS–PAGE)

Assiut Journal of Agricultural Sciences
Article 14, Volume 54, Issue 2, April 2023, Pages 190-201    PDF (536.96 K)
Document Type: Original Article
DOI: 10.21608/ajas.2023.187545.1221
Authors
Ahmed H. Ali* 1; Sayed A. Ahmed2; Gamal A.M. Abdu-Allah2; Soliman M. El-Sagheer1
1Plant Protection Department, Faculty of Agriculture, Al-Azhar University, Assiut 71524. Egypt
2Plant Protection Department, Faculty of Agriculture, Assiut University, Assiut. 71526 Egypt
Abstract
Although herbicides are important in controlling weeds in corn and sorghum and increasing their yields, some of these chemicals have severe side effects on the environmental components. These negative effects may be extended to crop seed quality. This  investigation was carried out with maize (Zea mays L.) and sorghum (Sorghum bicolor L.) in summer season of 2021 to study the effect of the recommended rate  of the following herbicides: (carfentrazon 1.5% + florasulam 0.5% + flurxypyr-methyl 14%), (carfentrazon 1.5% + florasulam 0.5% + flurxypyr-meptyl  14% + halosulfuron-methyl 75%), (tribenuron-methyl 16% + carfentrazon ethyl  12%), (tribenuron methyl  16% + carfentrazon ethyl 12% + halosulfuron-methyl 75%), (bromoxynil 20% + MCPA sodium 20%), (bromoxynil 20% + MCPA sodium 20% +  halosulfuron-methyl 75%), (halosulfuron-methyl 75%), (nicosulfuron 6%), nicosulfuron 6% + halosulfuron-methyl 75%), (foramsulfuron 2.25%), foramsulfuron 2.25% + halosulfuron-methyl 75%), comparing with the hand hoeing and the control on the seed proteins of commercial cultivars under Assiut field conditions. Results showed that all tested treatments gave different significant increases or decreases in seed protein profiles using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS–PAGE). Data obtained showed that the bands with different molecular weights (KDa) of Zea mays and Sorghum bicolor seeds might be related to the mode of action of herbicides used to control associated weeds in these crops. The present results suggested the possibility of applying (SDS-PAGE) as supportive method to determine the sub-lethal effects of herbicides on total protein in sorghum and maize seeds.
Keywords
Herbicides; Zea mays; Sorghum bicolor; SDS–PAGE
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