Quantification and risk assessment of Flutianil and of propiconazole in green bean by HPLC-DAD under greenhouse conditions | ||
| Egyptian Journal of Chemistry | ||
| Volume 66, Issue 13, December 2023, Pages 2149-2158 PDF (1.47 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2023.174226.7183 | ||
| Authors | ||
| El Desoky Ibrahim1; Dalia Ahmed Barakat* 2; Rania Mohamed Ahmed Helmy3 | ||
| 1Department of Economic Entomology and Pesticides, Faculty of Agriculture Cairo University | ||
| 2Department of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo University | ||
| 3Pesticide Residues and Environmental Pollution department, Central Agricultural Pesticide Laboratory, Agricultural Research Center,12618 Giza, EGYPT | ||
| Abstract | ||
| Green bean (Phaseolus vulgaris L.) is one of the important cash crops; which is subjected to many pests, consequently many pesticides are used. Flutianil as a novel compound and propiconazole as a reference compound, were sprayed at the recommended dose on green bean. Their residues and safety to humans under greenhouse conditions were evaluated. Samples were randomly collected at (2 h), 1, 3, 5, 7, 10, 15 and 21 days, respectively. Extraction was performed applying a quick, easy, cheap, effective, rugged, and safe approach (QuEChERS) to homogenized samples, coupled with HPLC-DAD for residues determination. Method was validated starting with blank samples, spiked at five levels (n = 6). Linearity was assessed by injections in triples (n=3) for five concentrations (0.01 to 10 mg kg -1 each), resulting in good linearity with regression coefficient (R2) 0.9956 and 0.9999, high accuracy, precision, matrix effect, satisfactory recoveries (76.9%–110.2%) and relative standard deviation (<20%). The limits of detection and quantification were 0.01 and 0.05 μg/kg, respectively. Pre-harvest intervals (PHIs) were 10 days and maximum residue limits (EU MRLs) was 0.01 for both pesticides. The assessment of health risk, based on dietary exposure, showed that green beans treated with both pesticides are safe to human. | ||
| Keywords | ||
| Green beans; Flutianil; propiconazole; residues; HPLC; risk assessment | ||
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