Protective Effect of Curcumin and Cerium Oxide Nanoparticles on Carboplatin Induced Myelotoxicity and Hepatotoxicity in Adult Male Wistar Rats | ||
| Zagazig Journal of Forensic Medicine and Toxicology | ||
| Article 4, Volume 21, Issue 2, July 2023, Pages 55-83 PDF (1.43 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/zjfm.2023.194806.1142 | ||
| Authors | ||
| Marwa Amer1; Dena Abdel Moawed* 2; Reham Sameh3; Rasha Ahmed Agaga4; Nisreen Elwany5; Amira Mohamed Abdelhamid6 | ||
| 1Forensic Medicine and Clinical Toxicology, Faculty of Medicine , Zagazig University | ||
| 2Forensic medicine and toxicology department Faculty of medicine Zagazig university | ||
| 3Pathology Department, Faculty of medicine, Zagazig University | ||
| 4Human anatomy and embryology, faculty of medicine, Zagazig university | ||
| 5Clinical pharmacy department, faculty of medicine, Zagazig university | ||
| 6Clinical pharmacology Department, Faculty of medicine, Zagazig University | ||
| Abstract | ||
| Background: Carboplatin is a chemotherapeutic agent used in many types of cancers. Carboplatin adversely affects multiple organs like the bone marrow, liver, gastrointestinal tract, and kidney. Aim of the study: The aim of this work is to evaluate the potential protective role of curcumin and cerium oxide nanoparticles (CONPs) on carboplatin induced myelotoxicity and hepatotoxicity. Methods: Forty-eight adult male Wistar rats were classified into seven groups; I. Control (Subdivided into negative and positive control groups), II. Curcumin-treated, III. CONPs-treated, IV. Carboplatin intoxicated, V. Curcumin-treated carboplatin intoxicated, VI. CONPs-treated carboplatin intoxicated & VII. Curcumin & CONPs-treated carboplatin intoxicated groups. Curcumin was administered orally, once daily for 4 consecutive weeks. CONPs, suspended in saline, and carboplatin were administered intraperitoneally once a week for 4 consecutive weeks.After 4 weeks, blood samples were collected after anesthesia of rats. The animals were then sacrificed, with bone marrow samples & livers collected for biochemical, histopathological and immunohistochemical studies. Results: Carboplatin treatment decreased blood cells count and elevated liver enzymes and bone marrow & hepatic malondialdehyde (MDA). It decreased GSH levels and causes DNA damage. Carboplatin induced hypocellularity in bone marrow samples and showed Strong 8-OHdG immunoreactivity. It also induced septal fibrosis with architectural distortion in liver tissue. Administration of either curcumin or CONPs ameliorated these toxic effects of carboplatin. Meanwhile the concurrent administration of curcumin and CONPs revealed much better improvement than each of them used alone. Conclusions: Combined administration of curcumin and CONPs ameliorated carboplatin-induced myelotoxicity and hepatotoxicity through antioxidant mechanism. | ||
| Keywords | ||
| Curcumin; Cerium oxide; Nanoparticles; Carboplatin; Myelotoxicity; Hepatotoxicity | ||
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